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首页> 外文期刊>Waste and biomass valorization >Continuous Fermentation of Wheat Straw Hydrolysate by Clostridium tyrobutyricum with In-Situ Acids Removal
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Continuous Fermentation of Wheat Straw Hydrolysate by Clostridium tyrobutyricum with In-Situ Acids Removal

机译：酪丁酸梭菌原位去除酸法连续发酵麦草水解液

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摘要

The present study focused on fermentative butyric acid production by Clostridium tyrobutyricum from pre-treated and hydrolysed wheat straw (PHWS) based on continuous operation mode and in situ acids extraction by reverse electro enhanced dialysis (REED). Different dilutions of PHWS in a synthetic medium (60-100 % v/v) were tested. It was found that continuous fermentation of PHWS greatly enhanced the sugar consumption rates and butyric acid productivity compared to batch tests, while application of REED enhanced them even further. Specifically, applying combined continuous operation mode and REED system for the fermentation of 70 % PHWS resulted in 19-and 53-fold higher glucose (1.37 g L~(-1) h~(-1)) and xylose (0.80 g L~(-1) h~(-1)) consumption rates, respectively, compared to those obtained by batch processing. Fermentation of 100 % PHWS continued unhindered with just urea and K_2HPO_4 added with butyric acid production rate, yield and selectivity being 1.30 g L~(-1) h~(-1), 0.45 g g~(-1) sugars and 0.88 g g~(-1) acids, respectively. These results were also confirmed in a 20 L pilot plant bioreactor system.
机译：本研究的重点是基于连续操作模式并通过逆电增强渗析（REED）原位提取酸，由预处理和水解的小麦秸秆（PHWS）生产的酪丁酸梭状芽孢杆菌发酵性丁酸。测试了在合成培养基（60-100％v / v）中PHWS的不同稀释度。结果发现，与批次试验相比，PHWS的连续发酵极大地提高了糖消耗率和丁酸生产率，而REED的应用甚至进一步提高了糖消耗率和丁酸生产率。具体而言，将连续操作模式和REED系统组合用于70％PHW​​S的发酵会产生19倍和53倍的葡萄糖（1.37 g L〜（-1）h〜（-1））和木糖（0.80 g L〜 （-1）h〜（-1））消耗率分别与通过批处理获得的消耗率相比。仅用尿素和添加丁酸的K_2HPO_4继续发酵100％PHW​​S，产量和选择性分别为1.30 g L〜（-1）h〜（-1），0.45 gg〜（-1）糖和0.88 gg〜 （-1）分别为酸。在20 L中试植物生物反应器系统中也证实了这些结果。

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  • 来源
    《Waste and biomass valorization》 |2015年第3期|317-326|共10页
  • 作者

    G. N. Baroi; I. V. Skiadas; P. Westermann; H. N. Gavala;

  • 作者单位

    Department of Chemistry and Bioscience, Section for Sustainable Biotechnology, Aalborg University (AAU), A C Meyers Vaenge 15, 2450 Copenhagen SV, Denmark;

    Department of Chemistry and Bioscience, Section for Sustainable Biotechnology, Aalborg University (AAU), A C Meyers Vaenge 15, 2450 Copenhagen SV, Denmark;

    Department of Chemistry and Bioscience, Section for Sustainable Biotechnology, Aalborg University (AAU), A C Meyers Vaenge 15, 2450 Copenhagen SV, Denmark;

    Department of Chemistry and Bioscience, Section for Sustainable Biotechnology, Aalborg University (AAU), A C Meyers Vaenge 15, 2450 Copenhagen SV, Denmark;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Butyric acid; Clostridium tyrobutyricum; Fermentation; Lignocellulosic biomass; Reverse electro enhanced dialysis; Wheat straw;

    机译：丁酸;酪丁酸梭菌;发酵;木质纤维素生物质;反向电增强透析;小麦秸秆;

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