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首页> 外文期刊>Wood Science and Technology >Analysis of DNA profiles of ash (Fraxinus excelsior L.) to provide evidence of illegal logging
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Analysis of DNA profiles of ash (Fraxinus excelsior L.) to provide evidence of illegal logging

机译：分析灰（Fraxinus excelsior L.）的DNA谱，以提供非法采伐的证据

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摘要

Illegal logging is a major problem in many European countries. Recent progress in molecular biology, however, has significantly improved the ability to accurately identify wood material. In this paper, the first application of microsatellite DNA markers is described in a case of illegal logging of European ash wood in Polish State Forests. The genetic fingerprints of seized ash wood samples were determined using six nuclear and four chloroplast microsatellite loci, characterized by sufficient stringency in forensic analyses. By comparing the DNA profiles obtained, the origin of one sample of ash wood used as evidence material was confirmed, from among three samples serving as reference material with 99.99999% of probability. This work demonstrates how DNA authenticity testing can serve as an important technical tool in monitoring the legality of the suspected ash timber and confirms the utility of these techniques in detecting illegally logged timber in general.
机译：在许多欧洲国家，非法采伐是一个主要问题。但是，分子生物学的最新进展显着提高了准确识别木质材料的能力。在本文中，描述了在波兰国家森林中非法砍伐欧洲白蜡木时微卫星DNA标记的首次应用。用六个核和四个叶绿体微卫星基因座确定了缉获的白蜡木样品的遗传指纹，其特征是法医分析具有足够的严格性。通过比较获得的DNA图谱，从用作参考材料的三个样品中确定了一个用作证据材料的白蜡木样品的起源，可能性为99.99999％。这项工作证明了DNA真实性测试如何可以用作监视可疑白蜡木合法性的重要技术工具，并证实了这些技术在检测一般非法采伐木材中的实用性。

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  • 来源
    《Wood Science and Technology》 |2017年第6期|1377-1387|共11页
  • 作者

    Tereba Anna; Woodward Stephen; Konecka Agata; Borys Magorzata; Nowakowska Justyna Anna;

  • 作者单位

    Forest Res Inst, Mol Biol Lab, 3 Braci Lesnej St, PL-05090 Sekocin Stary, Poland;

    Univ Aberdeen, Sch Biol Sci, Dept Plant & Soil Sci, Cruickshank Bldg, Aberdeen AB24 3UU, Scotland;

    Forest Res Inst, Mol Biol Lab, 3 Braci Lesnej St, PL-05090 Sekocin Stary, Poland;

    Forest Res Inst, Mol Biol Lab, 3 Braci Lesnej St, PL-05090 Sekocin Stary, Poland;

    Forest Res Inst, Mol Biol Lab, 3 Braci Lesnej St, PL-05090 Sekocin Stary, Poland;

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