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首页> 外文期刊>Pigment cell research >Reduced expression of vps11 causes less pigmentation in medaka, Oryzias latipes
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Reduced expression of vps11 causes less pigmentation in medaka, Oryzias latipes

机译：vps11的表达减少导致水aka（Myaka），长叶Oryzias latipes中的色素沉着减少

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In this article, we describe the medaka mutant pale gray eyes (pge) that shows reduction of black, white, and silver pigmentation and lethality approximately a week after hatching. The pge mutation was mapped to the tip of linkage group 14 and no recombinations were observed between the mutation and medaka vps11 in 900 meioses. Vps 11 is one of the evolutionarily conserved class C vacuolar protein sorting genes (c-vps: vps11, vps16, vps18, and vps33), whose products physically associate to form the c-vps protein complex required for vesicle docking and fusion in the budding yeast. Mutations in vps16, vps18, and vps33 are known to result in decreased pigmentation in organisms such as Drosophila. We cloned the full-length medaka vps11 cDNA by rapid amplification of cDNA ends (RACE) and found no RACE products from the pge mutants. Similarly, no vps11 transcripts were detected from the pge mutants by Northern analysis. The injection of an antisense morpholino against vps11 phenocopied the pge mutant. Taken together, the results suggest that reduced expression of medaka vps11 causes pge and that medaka vps11 is indispensable for survival and normal pigmentation.
机译：在本文中，我们描述了medaka突变体淡灰色眼睛（pge），它在孵化大约一周后显示出黑色，白色和银色色素沉着和致死率降低。 pge突变被定位到连锁群14的顶端，并且在900个meios中该突变与medaka vps11之间没有观察到重组。 Vps 11是进化上保守的C类液泡蛋白分选基因之一（c-vps：vps11，vps16，vps18和vps33），其产物物理缔合形成囊泡对接和融合中所需的c-vps蛋白复合物酵母。已知vps16，vps18和vps33中的突变会导致果蝇等生物体内色素沉着减少。我们通过快速扩增cDNA末端（RACE）克隆了全长medaka vps11 cDNA，发现没有来自pge突变体的RACE产物。类似地，通过Northern分析未从pge突变体中检测到vps11转录物。注射针对vps11的反义吗啉代可显着修饰pge突变体。两者合计，结果表明medaka vps11的表达减少会导致pge，而medaka vps11对于生存和正常色素沉着是必不可少的。

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