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Informatic and genomic analysis of melanocyte cDNA libraries as a resource for the study of melanocyte development and function

机译：黑色素细胞cDNA文库的信息学和基因组分析可作为研究黑色素细胞发育和功能的资源

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As part of the RIKEN mouse encyclopedia project, two cDNA libraries were prepared from melanocyte-derived cell lines, using techniques of full-length clone selection and subtractionormalization to enrich for rare transcripts. End sequencing showed that these libraries display over 83% complete coding sequence at the 5' end and 96-97% complete coding sequence at the 3' end. Evaluation of the libraries, derived from B16F10Y tumor cells and melan-c cells, revealed that they contain clones for a majority of the genes previously demonstrated to function in melanocyte biology. Analysis of genomic locations for transcripts revealed that the distribution of melanocyte genes is non-random throughout the genome. Three genomic regions identified that showed significant clustering of melanocyte-expressed genes contain one or more genes previously shown to regulate melanocyte development or function. A catalog of genes expressed in these libraries is presented, providing a valuable resource of cDNA clones and sequence information that can be used for identification of new genes important for melanocyte development, function, and disease.
机译：作为RIKEN小鼠百科全书计划的一部分，使用全长克隆选择和减法/归一化技术富集稀有的转录本，从黑素细胞衍生的细胞系中制备了两个cDNA文库。末端测序表明，这些文库在5'端显示超过83％的完整编码序列，在3'端显示96-97％的完整编码序列。对来自B16F10Y肿瘤细胞和黑色素c细胞的文库的评估显示，它们包含先前证明在黑色素细胞生物学中起作用的大多数基因的克隆。转录本的基因组位置分析表明，黑素细胞基因在整个基因组中的分布是非随机的。鉴定出三个显示黑素细胞表达基因显着聚集的基因组区域，包含一个或多个以前显示出调节黑素细胞发育或功能的基因。介绍了在这些文库中表达的基因目录，提供了宝贵的cDNA克隆和序列信息资源，可用于鉴定对黑素细胞发育，功能和疾病很重要的新基因。

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