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首页> 外文期刊>Pigment cell research >Keratinocyte cultures from involved skin in vitiligo patients show an impaired in vitro behaviour
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Keratinocyte cultures from involved skin in vitiligo patients show an impaired in vitro behaviour

机译：白癜风患者皮肤中的角质形成细胞培养显示出体外行为受损

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摘要

Vitiligo depigmentation is considered a consequence of either melanocyte disappearance or loss of functioning melanocytes in the involved areas. However, it has been reported that keratinocytes in involved vitiligo skin are damaged too. Based on this evidence, we evaluated the in vitro behaviour, in life span cultures, of involved and uninvolved vitiligo keratinocytes and their expression of proliferation, differentiation and senescence markers. An additional purpose was to investigate whether vitiligo keratinocytes from depigmented skin are able to sustain survival and growth of normal melanocytes (when added in co-culture experiments), as normal human keratinocytes manage to do. Our results demonstrate that almost all involved vitiligo keratinocytes have a shorter life span in vitro than the uninvolved cells and all of them do not maintain melanocytes in culture in a physiological ratio. Modification of proliferation and senescence marker expression also occurs. Indeed, we detected low initial expression levels of the senescence marker p16 in involved vitiligo keratinocytes, despite their shorter in vitro life span, and increased expression of proliferating cell nuclear antigen and p53. This preliminary analysis of a small number of in vitro cultured vitiligo keratinocytes suggests an impaired senescence process in lesional vitiligo keratinocytes and attempts to regulate it.
机译：白癜风的色素沉着被认为是受累区域黑色素细胞消失或功能性黑色素细胞丧失的结果。然而，据报道，涉及的白癜风皮肤中的角质形成细胞也被破坏。基于这一证据，我们评估了在生命培养中参与和未参与的白癜风角质形成细胞的体外行为，以及它们的增殖，分化和衰老标志物的表达。另一个目的是研究脱色皮肤的白癜风角质形成细胞是否能够像正常人角质形成细胞一样，维持正常黑素细胞的存活和生长（在共培养实验中添加时）。我们的结果表明，几乎所有受累的白癜风角质形成细胞在体外的寿命都比未受累的白细胞短，并且它们都不以生理比例维持培养中的黑素细胞。增殖和衰老标志物表达的修饰也发生。实际上，尽管它们的体外寿命较短，并且增殖细胞核抗原和p53的表达增加，但我们在参与的白癜风角质形成细胞中检测到了衰老标记p16的低初始表达水平。对少量体外培养的白癜风角质形成细胞的初步分析表明，病变白癜风角质形成细胞的衰老过程受损，并试图对其进行调节。

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