...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant and Soil >Direct measurement of roots in soil for single and mixed species using a quantitative DNA-based method
【24h】

Direct measurement of roots in soil for single and mixed species using a quantitative DNA-based method

机译：使用基于DNA的定量方法直接测量土壤中单个物种和混合物种的根

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Molecular techniques present a new opportunity to study roots and their interactions in soil. Extraction and quantification of species-specific DNA directly from soil allows direct identification of roots in mixed swards reducing the need for labour-intensive methods to recover and identify individual roots. DNA was extracted directly from up to 0.5 kg of soil and the presence of individual species quantified using species-specific probes with quantitative real-time PCR. A range of plant and soil factors influenced the DNA content measured in roots and it was necessary to account for these influences when converting DNA amount to root mass. The utility of the method for quantitative root studies was demonstrated in an experiment to investigate the effect of lime on root growth of acid-soil resistant and sensitive perennial grasses grown together in an aluminium-toxic soil. The root mass of an acid-soil resistant species was unaffected by lime application, whereas that of an acid-soil sensitive species was restricted by soil acidity. Molecular techniques present a promising tool for quantification of root mass directly in soil and have applications for field studies involving mixed species of plants.
机译：分子技术为研究根及其在土壤中的相互作用提供了新的机会。直接从土壤中提取和定量物种特异性DNA可以直接鉴定混合草皮中的根，从而减少了劳动强度大的方法来恢复和鉴定单个根的需要。直接从最多0.5千克的土壤中提取DNA，并使用具有定量实时PCR的物种特异性探针对单个物种的存在进行定量。一系列植物和土壤因素都会影响根中DNA含量，因此在将DNA量转换为根质量时必须考虑这些影响。在一项调查石灰对铝毒土壤中共同生长的耐酸碱和敏感多年生草根生长的实验中，证明了该方法可用于定量根研究。耐酸土壤树种的根质量不受石灰的影响，而耐酸土壤树种的根质量受土壤酸度的限制。分子技术为直接定量土壤中的根质量提供了一种有前途的工具，并已应用于涉及植物混合物种的田间研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号