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首页> 外文期刊>Chemical Engineering Research & Design: Transactions of the Institution of Chemical Engineers >Synthesis of crosslinked lipase aggregates and their use in the synthesis of aspirin
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Synthesis of crosslinked lipase aggregates and their use in the synthesis of aspirin

机译：交联脂肪酶聚集体的合成及其在阿司匹林合成中的应用

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摘要

Biocatalytic synthesis of drugs is imperative to avoid the hazardous environmental effects of the pharmaceutical production processes. In view of this, biocatalytic synthesis of aspirin is discussed in the present work. A crude lipase extracted from Geobacillus sp. was crosslinked using N,N-methylenebisacrylamide (N,N-MBAAm) and two different initiators: ammonium persulphate (APS) and gamma-irradiation. The crude lipase was crosslinked as such bypassing the separation procedures using a biocompatible crosslinker. The crosslinked lipase was characterized by various techniques and evaluated for its activity profile as a function of time, temperature, pH and substrate concentration and results were compared to its free form. The activity profile of the crosslinked lipase was observed to be only marginally lower than the free lipase. The crosslinked lipases were used as catalysts in the green synthesis of acetylsalicylic acid, aspirin, directly from the acetic acid. The reusability studies of the crosslinked lipases were carried out up to 10 cycles. (C) 2014 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
机译：药物的生物催化合成必须避免药物生产过程对环境的有害影响。鉴于此，在本工作中讨论了阿斯匹林的生物催化合成。从芽孢杆菌中提取的粗脂肪酶。使用N，N-亚甲基双丙烯酰胺（N，N-MBAAm）和两种不同的引发剂进行交联：过硫酸铵（APS）和伽马射线辐照。使用生物相容性交联剂绕过分离步骤将粗脂肪酶交联。交联的脂肪酶通过各种技术进行表征，并根据时间，温度，pH和底物浓度对其活性进行评估，并将结果与​​游离形式进行比较。观察到交联脂肪酶的活性谱仅略低于游离脂肪酶。交联的脂肪酶被用作直接由乙酸绿色合成乙酰水杨酸阿司匹林的催化剂。交联脂肪酶的可重复使用性研究最多进行了10个循环。 （C）2014化学工程师学会。由Elsevier B.V.发布。保留所有权利。

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