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首页> 外文期刊>Plant and Soil >ROOT SURFACE AREA MEASUREMENTS BASED ON ADSORPTION AND DESORPTION OF NITRITE
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ROOT SURFACE AREA MEASUREMENTS BASED ON ADSORPTION AND DESORPTION OF NITRITE

机译：基于亚硝酸盐吸附和脱附的根表面积测量

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摘要

A method, based on the negative adsorption of NO2- has been developed to determine surface area of roots. Young roots of 3-4 year old plants of Acacia catechu, Eucalyptus camaldulensis and Leucaena leucocephala were up-rooted and cut into 18 cylindrical pieces. Each root piece was immersed individually for 10 sec in 0.05 M, 0.10 M and 0.15 M aqueous NaNO2 solution and the excess solution on the root surface allowed to drain off. It was then transferred to conical flask containing distilled water and shaken for 15 min for desorption of nitrite. A known quantity of this aliquot was reacted with 1% acidic sulphanilamide and 0.02% NED HCl. A pink colour developed, and its optical density was read at 540 nm. A positive linear correlation was noted between colour density and root surface area. The respective correlation coefficient values for 0.05 M, 0.10 M and 0.15 M NaNO2 solutions were 0.973, 0.963 and 0.964 for Acacia catechu, 0.933, 0.903 and 0.898 for Eucalyptus camaldulensis and 0.968, 0.976 and 0.972 for Leucaena leucocephala (significant at p < 0.001). The method was successfully adopted to determine the root surface area of seedlings of Albizia lebbek, A. procera, Acacia auriculiformis, A. nilotica, Dalbergia latifolia and D. sissoo.
机译：已经开发出一种基于NO2-负吸附的方法来确定根的表面积。将3-4年生的相思树，桉树和白叶锦鸡儿的幼根连根拔起，切成18个圆柱体。将每个根片分别浸入0.05 M，0.10 M和0.15 M NaNO2水溶液中10秒钟，然后沥干根表面上的多余溶液。然后将其转移到装有蒸馏水的锥形瓶中，振摇15分钟以解吸亚硝酸盐。使已知量的该等分试样与1％的酸性磺酰胺和0.02％的NED HCl反应。出现粉红色，其光密度在540 nm处读取。在颜色密度和根表面积之间发现正线性相关。对于Naca2溶液，0.05 M，0.10 M和0.15 M NaNO2溶液的相关系数分别为：相思树为0.973、0.963和0.964，桉树为0.933、0.903和0.898，而白桦为0.968、0.976和0.972（p <0.001时显着） 。成功地采用该方法测定了白花槐，普罗旺斯，普罗旺斯，金合欢，尼罗罗非，阔叶黄檀和D. sissoo的幼苗的根表面积。

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