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首页> 外文期刊>Plant and Soil >Survival of rhizobia on arrowleaf clover seeds under stresses of seed-coat toxins, heat and desiccation.
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Survival of rhizobia on arrowleaf clover seeds under stresses of seed-coat toxins, heat and desiccation.

机译：在种皮毒素，热和干燥的压力下，根瘤菌在箭叶三叶草种子上的存活。

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摘要

Eighteen strains of Rhizobium leguminosarum bv. trifolii that had been found to be heat- and desiccation-stress tolerant in soil were tested for their resistance to Trifolium vesiculosum (arrowleaf clover) coat toxins using an agar plate technique and for survival on seed. They were further tested for their tolerance to seed coat toxins in combination with heat and desiccation stresses. The zone of inhibition in agar ranged from no inhibition to 21 mm diameter of growth inhibition around seed. Inoculation on seed under conditions of 28oC and 100% relative humidity for 7 days resulted in a range of survival from nearly 100% to less than 1%. The correlation between zone of growth inhibition and survival on seed was not statistically significant. Strains that were desiccation or heat tolerant in soil were not necessarily desiccation or heat tolerant on seed but strains that were heat tolerant in soil were better able to survive on glass beads at 37oC than strains were not heat tolerant in soil. Thezone of rhizobial inhibition on an agar medium was not a valid method to screen for isolates better able to survive on seed and the ability of rhizobia to withstand heat or drought stress on seed was not related to their ability to survive in soil.
机译：十八种豆科根瘤菌菌株。使用琼脂平板技术测试了在土壤中耐受热和干燥压力的三叶草对三叶草（箭叶三叶草）外壳毒素的抗性以及在种子上的存活率。进一步测试了它们对种皮毒素与热和干燥应力的结合的耐受性。琼脂中的抑制区范围从无抑制到种子周围生长抑制直径为21 mm。在28oC和100％相对湿度的条件下在种子上接种7天，存活率范围从近100％到小于1％。生长抑制区与种子存活之间的相关性在统计学上不显着。在土壤中具有干燥性或耐热性的菌株不一定在种子上具有干燥性或耐热性，但是在土壤中具有耐热性的菌株比在土壤中不具有耐热性的菌株能够更好地在37oC的玻璃珠上存活。琼脂培养基上的根瘤菌抑制区不是筛选能够更好地在种子上存活的分离株的有效方法，而根瘤菌在种子上承受高温或干旱胁迫的能力与其在土壤中存活的能力无关。

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