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首页> 外文期刊>Pigment cell research >Increased expression of integrins and decreased apoptosis correlate with increased melanocyte retention in cultured skin substitutes
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Increased expression of integrins and decreased apoptosis correlate with increased melanocyte retention in cultured skin substitutes

机译：整合素的表达增加和凋亡减少与培养的皮肤替代物中黑色素细胞保留增加有关

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Losses of human melanocytes (HM) in transplantation of cultured skin substitutes (CSS) may result from poor cellular attachments. To test this hypothesis, HM integrin expression was measured in four culture media: (a) melanocyte growth medium (MGM), an HM proliferation medium; (b) UCMC 160, a CSS maturation medium; (c) mMGM, modified MGM with 1.8 mM calcium; and (d) modified UCMC 160 with HM supplements (mUCMC 160). HM grew well in all media except UCMC 160. Increased expression of beta 1, beta 4, alpha 3 beta 1 and alpha 5 integrins on HM cultured in MGM and mMGM versus UCMC 160 was found by flow cytometry. Annexin V-allophycocyanin (APC) labeled HM in apoptosis and increased significantly in UCMC 160 (31.1%) compared with MGM (11.9%) or mMGM (13.9%). CSS were incubated in UCMC 160, mMGM or mUCMC 160 media, and grafted to athymic mice. In the mMGM group, grafts were darker as measured with a chromameter through 6 weeks and the average number of basal HM per field was greater at 12 weeks post-grafting. Increased graft loss was observed in the mMGM group which corresponded with the poor epidermal morphology in vitro. Although HM retention improved in vivo using mMGM to culture the CSS, the stability of the epidermis decreased. These results indicate that expression of integrins on HM in vitro correlates with HM retention in CSS and short-term survival after transplantation, but that long-term survival depends also on stable epithelium.
机译：培养的皮肤替代品（CSS）移植中人黑素细胞（HM）的丢失可能是由于细胞附着不良造成的。为了检验该假设，在四种培养基中测量了HM整联蛋白的表达：（a）黑色素细胞生长培养基（MGM），一种HM增殖培养基; （b）UCMC 160，一种CSS成熟介质; （c）mMGM，含1.8 mM钙的修饰MMG; （d）用HM补充剂（mUCMC 160）改良的UCMC 160。 HM在除UCMC 160以外的所有培养基中生长良好。通过流式细胞术发现，与UCMC 160相比，在MGM和mMGM中培养的HM上β1，β4，α3，β1和α5整联蛋白的表达增加。膜联蛋白V-藻蓝蛋白（APC）在凋亡中标记为HM，与MGM（11.9％）或mMGM（13.9％）相比，UCMC 160（31.1％）显着增加。将CSS在UCMC 160，mMGM或mUCMC 160培养基中孵育，然后移植到无胸腺小鼠中。在mMGM组中，用色谱仪测量到6周时，移植物变黑，并且在移植后12周，每个视野的平均基础HM数更大。在mMGM组中观察到移植物损失增加，这与体外不良的表皮形态有关。尽管使用mMGM培养CSS可以改善体内的HM保留率，但表皮的稳定性却下降了。这些结果表明，整合素在HM上的体外表达与HM在CSS中的保留和移植后的短期存活有关，但长期存活还取决于稳定的上皮细胞。

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