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首页> 外文期刊>Pigment cell research >Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83(+) teleost leukocyte cell line
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Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83(+) teleost leukocyte cell line

机译：黑素生成和黑素体运输到质膜的CD83（+）硬骨白细胞细胞系的证据。

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摘要

Visceral organs of ectothermic vertebrates harbour melanin-containing leukocytes termed melanomacrophages. These cells are thought to participate in immune reactions and free-radical trapping. In teleosts, the melanin-producing ability of melanomacrophages has hitherto not been confirmed by molecular techniques. Here, a leukocyte marker and the apparatus for melanosome production and transport were investigated in an Atlantic salmon (Salmo salar) pronephros-derived mononuclear leukocyte (SHK-1) cell line. The SHK-1 cells expressed transcripts specific for a mammalian CD83 homologue, a standard surface marker for activated or differentiated dendritic cells, and dopachrome tautomerase/tyrosinase-related protein-2, a melanocyte specific enzyme essential for melanin production. Reduction potential of melanin or its precursors was demonstrated histochemically after prolonged cultivation. Ultrastructural investigations revealed tyrosinase and acid phosphate activity in identical organelles and BSA-gold co-localized with multilamellar melanosomes after 2 h internalization. Apparently, melanosomes were transported and released through periodically occurring tubules fusing with the plasma membrane. Video monitoring revealed filopodia and macropinocytosis. These results showed that the SHK-1 cell line is capable of melanogenesis and melanosome secretion. Melanin-producing cells in teleost pronephros may represent a distinct CD83(+) leukocyte population consisting of phylogenetically relict multifunctional cells. This is the first report of a melanin-producing leukocyte cell-line.
机译：放热脊椎动物的内脏器官含有称为黑色素巨噬细胞的含黑色素的白细胞。这些细胞被认为参与免疫反应和自由基捕获。在硬骨鱼中，迄今为止尚未通过分子技术证实黑色素巨噬细胞产生黑色素的能力。在这里，研究了在大西洋鲑（Salmo salar）前食人来源的单核白细胞（SHK-1）细胞系中的白细胞标记物和用于生产黑素体的设备。 SHK-1细胞表达特异于哺乳动物CD83同源物的转录本，是活化或分化树突状细胞的标准表面标志物，以及多巴色素互变异构酶/酪氨酸酶相关蛋白2（一种对黑色素产生必不可少的黑素细胞特异性酶）。长期培养后在组织化学上证明了黑色素或其前体的还原潜力。超微结构研究显示，内化2小时后，相同细胞器中的酪氨酸酶和酸性磷酸酶活性以及与多层黑素体共定位的BSA-黄金。显然，黑素体通过与质膜融合的周期性出现的小管被运输和释放。视频监控显示丝状伪足和巨噬细胞增多。这些结果表明，SHK-1细胞系具有黑色素生成和黑素体分泌的能力。硬骨前体中产生黑色素的细胞可能代表了一个不同的CD83（+）白细胞群，由系统发育残留多功能细胞组成。这是产生黑色素的白细胞细胞系的首次报道。

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