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首页> 外文期刊>Plant and Soil >QTLs and epistasis underlying activity of acid phosphatase underphosphorus sufficient and deficient condition in rice (Oryza sativa L.)
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QTLs and epistasis underlying activity of acid phosphatase underphosphorus sufficient and deficient condition in rice (Oryza sativa L.)

机译:水稻充分和不足条件下酸性磷酸酶的QTL和上位性基础活性

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摘要

Eighty-four selected lines from a recombinant inbred (RI) line population of 284 lines derived from a cross between the indica varieties IR20 and IR55178-3B-9-3 were used in a hydroponic culture experiment with sufficient P supply (10 mg P L-1) and P-deficient stress (0.05 mg P L-1). After 2 weeks, the activity of acid phosphatase (AAP) in roots of each parent and each line from both normal culture and P-deficient stress was determined. QTLs for AAP, P-deficiency stress induced AAP (Psi-AAP) and relative AAP (RAAP) were detected using 178 molecular markers mapped on all 12 chromosomes based on single marker analysis and interval mapping. One QTL for AAP and three QTLs for Psi-AAP were detected on chromosome 1, 6 and 12, respectively. Two QTLs for RAAP were identical with these for Psi-AAP on chromosome 6 and 12. The results in this case indicated that the genetic system for Psi-AAP was different with that for AAP under normal culture. The AAP was mainly influenced by interaction among muti-factors, while Psi-AAP was controlled by a Psi genetic system.
机译:从in稻品种IR20和IR55178-3B-9-3杂交获得的284个重组自交(RI)品系群体中选择了84个品系用于水培培养实验中,磷的供应量充足(10 mg P L -1)和P缺乏应激(0.05 mg P L-1)。 2周后,测定正常培养和P缺乏胁迫下每个亲本和每个品系的根中酸性磷酸酶(AAP)的活性。基于单个标记分析和区间作图,使用映射在所有12条染色体上的178个分子标记,检测了AAP,P缺乏胁迫诱导的AAP(Psi-AAP)和相对AAP(RAAP)的QTL。在1号,6号和12号染色体上分别检测到一个AAP的QTL和三个Psi-AAP的QTL。 RAAP的两个QTL与6号和12号染色体上的Psi-AAP的QTL相同。在这种情况下,结果表明Psi-AAP的遗传系统与正常培养下AAP的遗传系统不同。 AAP主要受多因素相互作用的影响,而Psi-AAP受Psi遗传系统控制。

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