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首页> 外文期刊>Plant and Soil >Monitoring species of arbuscular mycorrhizal fungi in planta and in soilby nested PCR: application to the study of the impact of sewage sludge
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Monitoring species of arbuscular mycorrhizal fungi in planta and in soilby nested PCR: application to the study of the impact of sewage sludge

机译：巢式PCR监测植物和土壤中丛枝菌根真菌的种类：在污水污泥影响研究中的应用

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摘要

Nested PCR is a highly sensitive procedure for monitoring species of arbuscular mycorrhizal (AM) fungi and for determining their abundance in planta and in soil. DNA sequence variability in the D1 and D2 domains of the large ribosomal subunit is sufficient to design primers which discriminate between AM fungi at the species level. The usefulness of this molecular approach is illustrated in the present study on the differential impact of sewage sludges on a community of three AM fungi (Glomus mosseae, Glomus intraradices, Gigaspora rosea). Nested PCR was applied to trypan blue-stained mycorrhizal root fragments and soil mycelium from pot cultures of Medicago truncatula inoculated with the three fungi separately or together, and grown in sand containing sewage sludge that had been enriched or not with metallic or organic pollutants. G. intraradices and Gig. rosea varied in behaviour depending on whether they were inoculated alone or as a mixed community. G. mosseae showed a similar sensitivity towards each sewage sludge whether in community or alone, making it a potential candidate for ecotoxicological tests using M. truncatula to evaluate the quality or potential toxicity of sewage sludges which are widely used as fertilizers in agricultural lands.
机译：巢式PCR是一种高度敏感的程序，用于监视丛枝菌根（AM）真菌的种类并确定其在植物和土壤中的丰度。大核糖体亚基的D1和D2结构域中的DNA序列变异性足以设计出在物种水平上区分AM真菌的引物。本分子方法的有效性在污水污泥对三种AM真菌（Glomus mosseae，Glomus intraradices，Gigaspora rosea）群落的不同影响的研究中得到了说明。巢式PCR用于分别分离或一起接种了三种真菌的t藜苜蓿盆栽中的锥虫蓝染的菌根根碎片和土壤菌丝体，并在含污水污泥的沙子中生长，该污泥已被富集或未富集金属或有机污染物。 G. intraradices和Gig。 Rosea的行为各不相同，取决​​于它们是单独接种还是作为一个混合社区接种。 G. mosseae对每种污水污泥显示出相似的敏感性，无论是在社区还是单独的情况下，都使其成为潜在的候选物，可以使用truncatula分枝杆菌来评估被广泛用作农田肥料的污水污泥的质量或潜在毒性。

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