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The effects of nutrient supply, predominantly addition of iron, andrhizobial inoculation on the tolerance of Lupinus pilosus genotypes to acalcareous soil

机译:营养供应,主要添加铁和根瘤菌接种对羽扇豆基因型对缺钙土壤耐受性的影响

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Two glasshouse experiments were conducted to examine the effects of nutrient supply and rhizobial inoculation on the performance of Lupinus pilosus genotypes differing in tolerance to calcareous soils. In experiment 1, plants were grown for 84 days in a calcareous soil (50% CaCO3; soil water content 90% of field capacity) at four nutrient treatments (no-added nutrients, added nutrients without Fe, added nutrients with soil applied FeEDDHA, added nutrients with foliar applied FeSO4). In experiment 2, plants were grown for 28 days with supply of NH4NO3 without inoculation or inoculated with Bradyrhizobium sp. (Lupinus). Chlorosis in the youngest leaves was a good indicator of the relative tolerance of the genotypes to the calcareous soil in both experiments, except the treatment with FeEDDHA at 5 mg kg(-1) soil which was toxic to all genotypes. Chlorosis scores correlated with chlorophyll meter readings and chlorophyll concentrations. The foliar application of FeSO4 did not fully alleviate chlorotic symptoms despite concentrations of active or total Fe in the youngest leaves being increased. Adding nutrients and chemical nitrogen did not change the severity of chlorosis or improve the growth of the plant. The nutrient supply did not alter the ranking of tolerance of genotypes to the calcareous soil. The results suggest that nutrient deficiency or poor nodulation was not a major cause of poor plant growth on calcareous soils and that bicarbonate may exert a direct effect on chlorophyll synthesis. The mechanism for tolerance is likely to be related to an ability to exclude bicarbonate or prevent its transport to the leaves.
机译:进行了两个温室试验,以检验养分供应和根瘤菌接种对不同耐石灰性土壤的羽扇豆基因型性能的影响。在实验1中,在四种养分处理(不添加养分,不添加铁的养分,添加土壤的FeEDDHA的养分),石灰性土壤(50%CaCO3;土壤水含量的田间持水量的90%)中,植物生长了84天。叶面施用FeSO4添加营养。在实验2中,在不接种或未接种Bradyrhizobium sp的情况下,将植物生长28天,供应NH4NO3。 (羽扇豆)。在两个实验中,最年轻的叶片中的绿化作用是该基因型对钙质土壤相对耐受性的良好指标,除了在5 mg kg(-1)土壤中用FeEDDHA处理对所有基因型均具有毒性外。叶绿素得分与叶绿素仪读数和叶绿素浓度相关。尽管增加了最年轻叶片中活性或总Fe的浓度,但叶面施用FeSO4并不能完全缓解褪绿症状。添加营养素和化学氮并没有改变褪绿的严重程度或改善植物的生长。养分供应不会改变基因型对石灰性土壤的耐性等级。结果表明,营养不足或结瘤不良不是石灰性土壤上植物生长不良的主要原因,碳酸氢盐可能直接影响叶绿素的合成。耐受机制可能与排除碳酸氢根或阻止其转运到叶片的能力有关。

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