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首页> 外文期刊>Plant and Soil >The production and utilisation of monoclonal antibodies for identification of a Frankia strain utilised as inoculum for Casuarina equisetifolia
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The production and utilisation of monoclonal antibodies for identification of a Frankia strain utilised as inoculum for Casuarina equisetifolia

机译：单克隆抗体的生产和利用，用于鉴定用作木麻黄的接种物的Frankia菌株

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摘要

Monoclonal antibodies were raised against Frankia 0RS020607, a strain isolated originally by H. G. Diem from nodules of Casuarina equisetifolia from Senegal. One of these antibodies, mAb8C5, was shown by ELISA to have high, but not absolute specificity for 0RS020607. This antibody was employed to investigate the mobility and persistence of 0RS020607 in plantations of C. equisetifolia. Seedlings were inoculated in pots of sand in a forest nursery with 0RS020607, with local crushed nodule suspensions or were left uninoculated. They were planted out after 5 months in experimental plots on a moderately fertile black soil site and on a low organic, oxidised red soil site. Compared with crushed nodule inoculated seedlings or uninoculated controls, growth of seedlings at transplant was improved by inoculation with Frankia 0RS020607. However, 4 years after transplant to experimental plots, the growth of trees receiving different treatments was similar. The possibility that movement of ORS 020607 between treatment plots contributed to new nodulation and enhanced growth of uninoculated trees was tested using mAb8C5 in ELISA of Frankia mycelium, extracted from the nodules of trees of the three treatments. No significant differences in reactivity were detected between nodules from uninoculated and 0RS020607 inoculated trees at either the black or the red soil sites, showing that 0RS020607 moved between treatment plots at both sites. However, at both sites, nodules from plots of trees that were inoculated originally with local crushed nodules gave reactions in ELISA that were significantly lower than values for 0RS020607 inoculated trees, possibly due to the competitive effects for new nodulation of enhancement of the indigenous population of Frankia. Serological techniques using antibodies of high specificity against Frankia strains have value for rapid screening of field samples as a preliminary for further analysis by more discriminatory techniques based on assays of genetic polymorphisms.
机译：产生了针对Frankia 0RS020607的单克隆抗体，该菌株最初由H. G. Diem从塞内加尔的Casuarina equisetifolia结节中分离出来。 ELISA显示这些抗体之一mAb8C5对0RS020607具有很高的特异性，但不是绝对特异性。该抗体被用于研究0RS020607在马鞭毛衣原体人工林中的迁移和持久性。在森林育苗场中用0RS020607将幼苗接种在沙罐中，并用局部压碎的根瘤悬浮液接种或不接种。 5个月后将它们种植在中等肥沃的黑土和低有机氧化红土的试验田中。与粉碎的结节接种的幼苗或未接种的对照相比，接种Frankia 0RS020607可以改善移植时幼苗的生长。但是，在移植到实验区的4年后，接受不同处理的树木的生长是相似的。使用mAb8C5在Frankia菌丝体的ELISA中测试了从三个处理的树木结节提取的ORS 020607在处理地块之间移动有助于新结瘤和未接种树的生长的可能性。在黑色或红色土壤位点，未接种树和0RS020607接种树的根瘤之间未检测到反应性的显着差异，表明0RS020607在两个位置的处理区之间移动。但是，在这两个站点上，最初用局部压实结核接种的树丛中的结核在ELISA中的反应均显着低于0RS020607接种树木的值，这可能是由于新的结瘤促进了本地种群数量增加的竞争效应。法兰克使用针对Frankia菌株的高特异性抗体的血清学技术具有快速筛选田间样品的价值，可作为通过基于遗传多态性分析的更具区分性的技术进行进一步分析的基础。

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