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Cell wall-degrading enzymes and aggressiveness in Stagonospora nodorum.

机译：Stagonospora nodorum中的细胞壁降解酶和攻击性。

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摘要

Stagonospora nodorum [Phaeosphaeria nodorum, syn. Leptosphaeria nodorum] produces cell wall-degrading enzymes when grown in culture media containing cell wall components. The pathogen grew as well on minimal agar plates containing cellulose, xylan and pectin, but produced sparser mycelia. Four cell wall-degrading enzymes, cellulase, xylanase [xylan endo-1,3- beta -xylosidase], pectinase [polygalacturonase] and beta -1,3-glucanase, were coordinately induced in culture filtrates growing on xylan and cellulose as substrates. An aggressive isolate (sn26-1) secreted more cell wall-degrading enzymes than the others. Based on isoelectric focusing profiles, six to seven xylanase isoenzymes were induced by cellulose and xylan. No difference was found in the high (sn26-1) and low (9074) aggressive isolates. Addition of cell wall-degrading enzyme mixtures, not high xylanase alone, to a spore suspension of a low aggressive isolate (9074) caused a limited increase in tissue necrosis. We conclude that the cell wall-degrading enzymes play a role in early penetration of the host by the fungus, but they are not important elicitors for disease development..
机译：Stagonospora nodorum [Phoeosphaeria nodorum，syn。当在含有细胞壁成分的培养基中生长时，Noepteptopheria nodorum]会产生细胞壁降解酶。病原体在含有纤维素，木聚糖和果胶的最小琼脂平板上也生长，但产生了稀疏的菌丝体。在以木聚糖和纤维素为底物生长的培养滤液中，协同诱导了四种细胞壁降解酶，纤维素酶，木聚糖酶[木聚糖内切1,3-β-木糖苷酶]，果胶酶[聚半乳糖醛酸酶]和β-1,3-葡聚糖酶。侵略性分离株（sn26-1）分泌的细胞壁降解酶比其他分离物更多。基于等电聚焦曲线，纤维素和木聚糖可诱导六到七个木聚糖酶同工酶。在高（sn26-1）和低（9074）侵袭性分离株中未发现差异。将细胞壁降解酶混合物，而不是仅单独的高木聚糖酶，添加到低侵蚀性分离株的孢子悬浮液中（9074），导致组织坏死的增加有限。我们得出的结论是，细胞壁降解酶在真菌对宿主的早期渗透中起作用，但是它们不是疾病发展的重要诱因。

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