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首页> 外文期刊>Plant Breeding and Seed Science >Androgenic potential of Polish barley cultivars in anther culture in vitro.
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Androgenic potential of Polish barley cultivars in anther culture in vitro.

机译：波兰大麦品种在体外花药培养中的雄激素潜力。

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摘要

This study evaluated the androgenic potential of 21 Polish cultivars of spring and winter barley. The effects of different culture factors, including pre-treatment, medium and plant growth regulators, were evaluated. The cultivars exhibited different androgenic potential. On average, less than 10 green plants were obtained from 100 anthers (isolated from 3 spikes). Cold pre-treatment of spikes favoured the formation of embryogenic structures (embryos/calluses), most especially in winter cultivars, whereas mannitol pre-treatment enhanced the development of green plants, particularly in spring cultivars. The first embryogenic structures that formed on the induction medium in 4 weeks of culture had the greatest ability to develop green plants, while embryos/calluses collected after 8 weeks of culture were the least efficient. FHG medium supplemented with 1 mg BAP [benzyladenine]/litre and 0.2 mg 2,4-D/litre was optimum for the induction of androgenesis, while the same medium supplemented with 0.4 mg BAP/litre and 3% maltose was optimum for plant development..
机译：这项研究评估了21个波兰大麦春季和冬季大麦的雄激素潜力。评估了不同培养因子的影响，包括预处理，培养基和植物生长调节剂。品种表现出不同的雄激素潜力。平均而言，从100株花药中获得不到10株绿色植物（从3个穗中分离）。穗的冷预处理有利于胚发生结构（胚/愈伤组织）的形成，特别是在冬季栽培品种中，而甘露醇预处理可以促进绿色植物的发育，特别是在春季栽培品种中。培养4周后在诱导培养基上形成的第一个胚发生结构具有最大的发展绿色植物的能力，而培养8周后收集的胚/愈伤组织效率最低。补充1 mg BAP [苄基腺嘌呤] /升和0.2 mg 2,4-D /升的FHG培养基最适合诱导雄激素生成，而添加0.4 mg BAP /升和3％麦芽糖的相同培养基最适合植物发育..

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