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首页> 外文期刊>Plant Cell Reports >Effects of 2-aminoindan-2-phosphonic acid treatment on the accumulation of salidroside and four phenylethanoid glycosides in suspension cell culture of Cistanche deserticola
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Effects of 2-aminoindan-2-phosphonic acid treatment on the accumulation of salidroside and four phenylethanoid glycosides in suspension cell culture of Cistanche deserticola

机译：2-氨基茚满-2-膦酸处理对肉stan蓉肉悬液培养中红景天苷和四种苯基乙醛糖苷积累的影响

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摘要

2-Aminoindan-2-phosphonic acid (AIP), a specific competitive phenylalanine ammonia lyase (PAL) inhibitor was applied to a suspension cell culture of Cistanche deserticola. The effects of AIP treatment on cell growth, PAL activity, contents and yields of total phenolic compound, salidroside and four phenylethanoid glycosides (PheGs) are investigated. The results demonstrated that, 0.5 and 2.0 oM AIP treatments had similar effects on the measurements investigated in this study. AIP treatment resulted in significant decreases in PAL activity, total phenolic compounds content, and PheGs content. Linear regression analysis showed that PAL activity had a high correlation coefficient with the total phenolic compound content and the four PheGs contents. Total PAL activity-time area under curve (AUC) had a high correlation coefficient with the total phenolic compound yield and the yields of five tested compounds in untreated cell samples. In AIP-treated cells, total PAL activity-time AUC retained a high correlation with the total phenolic compound yield and the yields of three tested compounds, echinacoside, acteoside, and tubuloside A, but not salidroside and cistanoside A. The difference could be caused by the different biosynthetic origins of each of the tested compounds. These results demonstrate the important role of PAL in the biosynthesis of PheGs in the suspension cell culture of C. deserticola.
机译：2-Aminoindan-2-phosphonic acid（AIP），一种特殊的竞争性苯丙氨酸氨裂合酶（PAL）抑制剂，被用于肉Ci蓉的悬浮细胞培养。研究了AIP处理对细胞生长，PAL活性，总酚类化合物，红景天苷和四种苯乙醛糖苷（PheGs）的含量和产量的影响。结果表明，在本研究中，0.5和2.0 oM AIP处理对测量值具有相似的影响。 AIP处理导致PAL活性，总酚类化合物含量和PheGs含量显着下降。线性回归分析表明，PAL活性与总酚类化合物含量和四种PheGs含量具有较高的相关系数。总PAL活性-时间曲线下面积（AUC）与总酚类化合物的收率和未经处理的细胞样品中5种受试化合物的收率具有较高的相关系数。在经AIP处理的细胞中，总PAL活性时间AUC与总酚类化合物的收率以及三种测试化合物（紫锥花苷，Acteoside和tubuloside A）的产量高度相关，但与红景天苷和Cistanoside A却没有高度相关性。通过每种测试化合物的不同生物合成来源。这些结果证明了PAL在C.desticicola悬浮细胞培养物中PheGs的生物合成中的重要作用。

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