• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell Reports >Flavonoid production in transgenic hop (Humulus lupulus L.) altered by PAP1/MYB75 from Arabidopsis thaliana L.
【24h】

Flavonoid production in transgenic hop (Humulus lupulus L.) altered by PAP1/MYB75 from Arabidopsis thaliana L.

机译：拟南芥（Arabidopsis thaliana L.）的 PAP1 / MYB75 改变了转基因啤酒花（ L.）的类黄酮生成。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hop is an important source of secondary metabolites, such as flavonoids. Some of these are pharmacologically active. Nevertheless, the concentration of some classes as flavonoids in wild-type plants is rather low. To enhance the production in hop, it would be interesting to modify the regulation of genes in the flavonoid biosynthetic pathway. For this purpose, the regulatory factor PAP1/AtMYB75 from Arabidopsis thaliana L. was introduced into hop plants cv. Tettnanger by Agrobacterium-mediated genetic transformation. Twenty kanamycin-resistant transgenic plants were obtained. It was shown that PAP1/AtMYB75 was stably incorporated and expressed in the hop genome. In comparison to the wild-type plants, the color of female flowers and cones of transgenic plants was reddish to pink. Chemical analysis revealed higher levels of anthocyanins, rutin, isoquercitin, kaempferol-glucoside, kaempferol-glucoside-malonate, desmethylxanthohumol, xanthohumol, alpha-acids and beta-acids in transgenic plants compared to wild-type plants. copyright Springer-Verlag 2011.
机译：啤酒花是次生代谢产物（如黄酮类）的重要来源。其中一些具有药理活性。然而，野生类植物中某些类黄酮的浓度相当低。为了提高啤酒花的产量，在类黄酮生物合成途径中修饰基因的调控将是有趣的。为此，将拟南芥（Arabidopsis thaliana L.）的调节因子 PAP1 / AtMYB75 引入啤酒花植物cv中。农杆菌介导的遗传转化。获得了二十种抗卡那霉素的转基因植物。结果表明， PAP1 / AtMYB75 在啤酒花基因组中稳定整合并表达。与野生型植物相比，雌花和转基因植物的视锥细胞的颜色从红色变为粉红色。化学分析显示，与野生型植物相比，转基因植物中花青素，芦丁，异槲皮苷，山emp酚-葡萄糖苷，山emp酚-葡萄糖苷-丙二酸酯，去甲基黄腐酚，黄腐酚，α-酸和β-酸的含量更高。版权所有Springer-Verlag 2011。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号