...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell Reports >Expression of the cholera toxin B subunit (CT-B) in maize seeds and a combined mucosal treatment against cholera and traveler's diarrhea. (Special Issue: Plant molecular pharming 2012 and beyond.)
【24h】

Expression of the cholera toxin B subunit (CT-B) in maize seeds and a combined mucosal treatment against cholera and traveler's diarrhea. (Special Issue: Plant molecular pharming 2012 and beyond.)

机译：霍乱毒素B亚基（CT-B）在玉米种子中的表达以及针对霍乱和旅行者腹泻的联合粘膜治疗。 （特刊：2012年及以后的植物分子药物开发。）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The non-toxic B subunit (CT-B) of cholera toxin from Vibrio cholerae is a strong immunogen and amplifies the immune reaction to conjugated antigens. In this work, a synthetic gene encoding for CT-B was expressed under control of a gamma -zein promoter in maize seeds. Levels of CT-B in maize plants were determined via ganglioside dependent ELISA. The highest expression level recorded in T1 generation seeds was 0.0014% of total aqueous soluble protein (TASP). Expression level of the same event in the T2 generation was significantly increased to 0.0197% of TASP. Immunogenicity of maize derived CT-B was evaluated in mice with an oral immunization trial. Anti-CTB IgG and anti-CTB IgA were detected in the sera and fecal samples of the orally immunized mice, respectively. The mice were protected against holotoxin challenge with CT. An additional group of mice was administrated with an equal amount (5 micro g per dose each) of mixed maize-derived CT-B and LT-B (B subunit of E. coli heat labile toxin). In the sera and fecal samples obtained from this group, the specific antibody levels were enhanced compared to either the same or a higher amount of CT-B alone. These results suggest that a synergistic action may be achieved using a CT-B and LT-B mixture that can lead to a more efficacious combined vaccine to target diarrhea induced by both cholera and enterotoxigenic strains of Escherichia coli.
机译：霍乱弧菌霍乱毒素的无毒B亚基（CT-B）是一种强大的免疫原，可增强针对结合抗原的免疫反应。在这项工作中，在玉米种子中，在γ-玉米醇溶蛋白启动子的控制下表达了编码CT-B的合成基因。通过神经节苷脂依赖性ELISA确定玉米植物中CT-B的水平。在T 1 种子中记录到的最高表达水平是总水溶性蛋白（TASP）的0.0014％。 T 2 世代中同一事件的表达水平显着提高至TASP的0.0197％。通过口服免疫试验在小鼠中评估了玉米衍生的CT-B的免疫原性。在口服免疫小鼠的血清和粪便样品中分别检测到抗CTB IgG和抗CTB IgA。用CT保护小鼠免受全毒素攻击。给予另一组小鼠等量的玉米衍生的CT-B和LT-B（大肠杆菌热不稳定毒素的B亚基）（每剂量5微克）。在从该组获得的血清和粪便样品中，与单独的相同数量或更高数量的CT-B相比，特异性抗体水平有所提高。这些结果表明，使用CT-B和LT-B混合物可以实现协同作用，从而导致更有效的联合疫苗靶向由霍乱和大肠杆菌产生的肠毒素菌株引起的腹泻。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号