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首页> 外文期刊>Plant Cell Reports >Characterization and expression of an nsLTPs-like antimicrobial protein gene from motherwort (Leonurus japonicus)
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Characterization and expression of an nsLTPs-like antimicrobial protein gene from motherwort (Leonurus japonicus)

机译：益母草（Leonurus japonicus）的nsLTPs样抗菌蛋白基因的表征和表达

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摘要

In screening for potent antimicrobial proteins (AMPs) from plant seeds, we had purified a heat-stable AMP, LJAMP2, from the seeds of a medicine herb, motherwort (Leonurus japonicus Houtt). In an in vitro assay, the protein can inhibit the growth of both fungi and bacteria. Then a cDNA encoding LJAMP2 was cloned by the rapid amplification of cDNA ends based on the N-terminal amino acid sequence determined. The deduced amino acid sequences of this cDNA show similarity to plant non-specific lipid transfer proteins. Northern blotting assay revealed that this nsLTP-like gene, designated LJAMP2, was expressed in seeds. Overexpression of LJAMP2 in tobacco enhanced resistance to the fungal pathogen Alternaria alternata and the bacterial pathogen Ralstonia solanacearum, significantly, while no visible alteration in plant growth and development. Our data confirm the antifungal and antibacterial function of LJAMP2 from motherwort seeds and suggest the potential of LJAMP2 in improving disease resistance in plants.
机译：在从植物种子中筛选有效的抗菌蛋白（AMPs）时，我们从药草益母草（Leonurus japonicus Houtt）的种子中纯化了热稳定的AMP LJAMP2。在体外测定中，蛋白质可以抑制真菌和细菌的生长。然后，基于所确定的N末端氨基酸序列，通过快速扩增cDNA末端来克隆编码LJAMP2的cDNA。该cDNA的推导的氨基酸序列显示出与植物非特异性脂质转移蛋白的相似性。 Northern印迹分析显示该nsLTP样基因，称为LJAMP2，在种子中表达。烟草中LJAMP2的过表达显着增强了对真菌病原体链格孢和细菌病原体Ralstonia solanacearum的抗性，而在植物生长和发育中没有可见的变化。我们的数据证实了来自益母草种子的LJAMP2的抗真菌和抗菌功能，并暗示了LJAMP2在改善植物抗病性方面的潜力。

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