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Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones

机译：低温保存的木瓜无性系的田间性能评估和遗传完整性评估

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This paper is the first report of field performance and evaluation of morphological traits following cryopreservation in four genotypes of Carica papaya (Z6, 97, TS2 and 35). It also describes the successful establishment of in vitro plantlets following vitrification-based cryopreservation of shoot tips and their acclimatisation through to field establishment. Cloned plants resulting from untreated controls, as well as controls taken at three other stages of the cryopreservation process (dissection, pre-treatment, plant vitrification solution 2 (PVS2) treatment) and cryopreserved plants were established to ensure a rigorous appraisal of any variation. Results indicate no differences between any of the control plants or cryopreserved plants for either growth performance or morphology. In addition, both randomly amplified DNA fingerprinting and amplified DNA methylation polymorphism markers were used to assess any genomic or methylation changes in genotype 97 at four different developmental stages post cryopreservation (in vitro, acclimatisation and field). Only small genomic DNA modifications (0-8.3%) were detected in field stage plants and methylation modifications (0-4.3%) were detected at both the in vitro and field stages for samples treated with PVS2 or cryopreservation.
机译：本文是四种番木瓜（Z6、97，TS2和35）基因型在低温保存后的田间表现和形态性状评价的首次报道。它还描述了基于玻璃化的芽尖冷冻保存及其在田间建立之前的适应性，成功建立了体外小植株。建立了未经处理的对照以及在冷冻保存过程的其他三个阶段（解剖，预处理，植物玻璃化溶液2（PVS2）处理）采取的对照的克隆植物和冷冻保存的植物，以确保对任何变异进行严格评估。结果表明，在任何对照植物或低温保存的植物之间，无论是生长性能还是形态上都没有差异。另外，随机扩增的DNA指纹图谱和扩增的DNA甲基化多态性标记物均用于评估冷冻保存后四个不同发育阶段（体外，适应和田间）基因型97的任何基因组或甲基化变化。在田间植物中仅检测到小的基因组DNA修饰（0-8.3％），而在体外和田间阶段，对于用PVS2或冷冻保存的样品，仅检测到甲基化修饰（0-4.3％）。

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