...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell Reports >Isolation of DNA-methyltransferase genes from strawberry (Fragaria x ananassa Duch.) and their expression in relation to micropropagation
【24h】

Isolation of DNA-methyltransferase genes from strawberry (Fragaria x ananassa Duch.) and their expression in relation to micropropagation

机译：草莓（Fragaria x ananassa Duch。）DNA-甲基转移酶基因的分离及其与微繁殖的关系

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

DNA methylation can control gene expression and may also play a role in plant development. Methylation of cytosine residues in DNA is enzymatically catalyzed by DNA methyltransferases. In this study, full-length genomic genes and cDNAs of methyltransferase (MET1) and domain-rearranged methyltransferase (DRM) were isolated from strawberry (Fragaria x ananassa Duch.). Two genomic clones (FaMET1a and FaMET1b) encoding MET1 had open-reading frame of 4,695 and 4,671 nucleotides with two introns, respectively. Amino acid sequence comparison indicated high similarity (98.72% identity) of strawberry MET1 protein to other plant MET1 sequences. The full-length cDNA of strawberry DRM genes (FaDRMa, FaDRMb and FaDRMc) were 2,273, 2,282 and 2,288 bp, respectively. Ten introns with different sizes were dispersed in FaDRM genes. Similarly, FaDRMa, FaDRMb and FaDRMc had high-sequence similarity overall. Expressions of strawberry MET1 and DRM genes were compared among in vitro-micropropagated plants, generations of micropropagated plants and conventionally propagated plants. The transcriptional expressions of both FaMET1 and FaDRM genes were downregulated in micropropagated plants, and they were recovered in the first and second runner generations of micropropagated plants. However, there was a slighter difference in global DNA methylation rates between micropropagated plants and conventionally propagated plants. Therefore, there was no positive relation between global DNA methylation rates and the expression levels of MET1 and DRM genes.
机译：DNA甲基化可以控制基因表达，也可能在植物发育中起作用。 DNA中的胞嘧啶残基的甲基化被DNA甲基转移酶催化。在这项研究中，从草莓（Fragaria x ananassa Duch。）中分离出了甲基转移酶（MET1）和域重排的甲基转移酶（DRM）的全长基因组基因和cDNA。编码MET1的两个基因组克隆（FaMET1a和FaMET1b）分别具有两个内含子，分别具有4,695和4,671个核苷酸的开放阅读框。氨基酸序列比较表明草莓MET1蛋白与其他植物MET1序列具有高度相似性（98.72％同一性）。草莓DRM基因（FaDRMa，FaDRMb和FaDRMc）的全长cDNA分别为2,273、2,282和2,288 bp。十个不同大小的内含子分散在FaDRM基因中。同样，FaDRMa，FaDRMb和FaDRMc总体上具有高序列相似性。在体外微繁殖植物，微繁殖植物世代和常规繁殖植物之间比较了草莓MET1和DRM基因的表达。 FaMET1和FaDRM基因的转录表达在微繁殖植物中均被下调，并在微繁殖植物的第一代和第二代转基因中被回收。但是，微繁殖植物和常规繁殖植物之间的总体DNA甲基化速率略有不同。因此，全球DNA甲基化率与MET1和DRM基因的表达水平之间没有正相关关系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号