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首页> 外文期刊>Plant Cell Reports >Cell wall polysaccharide distribution in Miscanthus lutarioriparius stem using immuno-detection. (Special issue: Plant science and biotechnology in China (Volume I).)
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Cell wall polysaccharide distribution in Miscanthus lutarioriparius stem using immuno-detection. (Special issue: Plant science and biotechnology in China (Volume I).)

机译：利用免疫检测方法，在芒草（Miscanthus lutarioriparius）茎中细胞壁多糖分布。 （特刊：中国植物科学与生物技术（第一卷）。）

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摘要

Deposition and modification of cell wall polysaccharides during stem development affect biomass yield of the Miscanthus energy crop. The distribution patterns of cell wall polysaccharides in the 2nd and the 11th internodes of M. lutarioriparius stem were studied using in situ immunofluorescence assay. Crystalline cellulose and xylan were present in most of the stem tissues except phloem, where xyloglucan was the major composition of hemicellulose. The distribution of pectin polysaccharides varied in stem tissues, particularly in vascular bundle elements. Xylogalacturonan, feruloylated-1,4- beta -d-galactan and (1,3)(1,4)- beta -glucans, however, were insufficient for antibodies binding in both internodes. Furthermore, the distribution of cell wall polysaccharides was differentiated in the two internodes of M. lutarioriparius. The significant differences in the pattern of occurrence of long 1,5- alpha -l-arabinan chain, homogalacturonan and fucosylated xyloglucans epitope were detected between the two internodes. In addition, the relationships between probable functions of polysaccharides and their distribution patterns in M. lutarioriparius stem cell wall were discussed, which would be helpful to understand the growth characteristics of Miscanthus and identify potential targets for either modification or degradation.
机译：茎发育过程中细胞壁多糖的沉积和修饰影响芒草能源作物的生物量产量。利用原位免疫荧光分析技术研究了lutarioriparius茎第二节和第十一节细胞壁多糖的分布规律。结晶纤维素和木聚糖存在于除韧皮部以外的大多数茎组织中，其中木葡聚糖是半纤维素的主要成分。果胶多糖在茎组织中的分布各不相同，特别是在血管束成分中。然而，木葡糖醛酸聚糖，阿魏酰化的1,4-β-d-半乳聚糖和（1,3）（1,4）-β-葡聚糖对两个节点间的抗体结合均不足。此外，细胞壁多糖的分布在M. lutarioriparius的两个节间有所区别。在两个节间之间检测到长的1，5-α-1-阿拉伯聚糖链，同型半乳糖醛酸和岩藻糖基化木葡聚糖表位的发生模式的显着差异。此外，还讨论了多糖可能的功能与其在毛状支原体干细胞壁中的分布模式之间的关系，这有助于了解芒草的生长特性，并确定修饰或降解的潜在靶标。

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