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首页> 外文期刊>Plant Disease >Early detection of Leifsonia xyli subsp. xyli in sugarcane leaves by real-time polymerase chain reaction.
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Early detection of Leifsonia xyli subsp. xyli in sugarcane leaves by real-time polymerase chain reaction.

机译：早期发现Leifsonia xyli亚种。甘蔗叶中的木糖通过实时聚合酶链反应。

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摘要

A real-time, polymerase chain reaction (PCR) assay was developed for detecting Leifsonia xyli subsp. xyli in sugarcane leaf tissue. Real-time PCR assays were conducted on the youngest, fully expanded leaf of three cultivars collected bi-weekly from field nurseries between 11 April and 19 July 2005. L. xyli subsp. xyli infection was detected in leaves collected at all sampling dates, including those from 1-month-old plants on 11 April. Assays conducted on older, more rapidly growing plants (28 July and 21 October 2005) indicated that leaf position affects assay efficiency. Conventional PCR was less efficient than real-time PCR for detecting L. xyli subsp. xyli in leaf tissue. Real-time PCR was used to rank cultivars for susceptibility to L. xyli subsp. xyli infection based on the relative titer of L. xyli subsp. xyli in leaves of inoculated, 3- and 4-month-old greenhouse-grown plants. The ranking of cultivars by real-time PCR was in close agreement with the ranking determined by tissue-blot enzyme immunoassay performed on tissue from 7- to 9-month-old stalks..
机译：开发了一种实时聚合酶链反应（PCR）检测试剂盒，用于检测Xyli亚种。甘蔗叶组织中的木糖。在2005年4月11日至7月19日之间，每两周从田间苗圃中采集的三个品种的最年轻，完全展开的叶片上进行实时PCR分析。在所有采样日期采集的叶片中均检测到了木聚糖感染，包括4月11日1个月大植物的叶片中。在较早，生长较快的植物上进行的测定（2005年7月28日和10月21日）表明，叶片位置会影响测定效率。传统的PCR检测木糖乳杆菌亚种的效率不如实时PCR。叶组织中的木糖。实时PCR被用于对变种对木糖乳杆菌亚种的敏感性进行分级。基于木糖乳杆菌亚种的相对滴度的木糖感染。接种后的3个月和4个月大的温室植物的叶片中的木糖。通过实时PCR对品种的排名与通过对7个月至9个月大的茎组织进行组织印迹酶免疫测定法确定的排名非常吻合。

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