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首页> 外文期刊>Plant Disease >First report of Iris yellow spot virus infecting onion in Kenya and Uganda.
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First report of Iris yellow spot virus infecting onion in Kenya and Uganda.

机译：在肯尼亚和乌干达首次报道了鸢尾黄斑病毒感染洋葱的报道。

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摘要

Iris yellow spot virus (IYSV), transmitted by Thrips tabaci, is an economically important viral pathogen of bulb and seed onion crops in many onion-growing areas of the world. In Africa, IYSV has been reported in Reunion and South Africa. In September 2009, symptoms suspected to be caused by IYSV were observed on onions and leeks cultivated in Nairobi, Kenya. Symptoms consisted of spindle-shaped, straw-coloured, irregular chlorotic lesions with occasional green islands on the leaves. The presence of the virus was confirmed with IYSV-specific Agdia Flash Kits. Subsequently, surveys were undertaken in small-holder farms in onion production areas of Makueni (January 2010) and Mwea (August 2010) in Kenya and Kasese (January 2010) and Rwimi (January 2010) in Uganda. The incidence of disease in these locations ranged between 27 and 72%. Onion leaves showing symptoms of IYSV infection collected from both locations tested positive for the virus by double-antibody sandwich-ELISA with IYSV-specific antiserum. This was confirmed by reverse transcription-PCR with primers. This is thought to be the first report of IYSV infecting onion in Kenya and Uganda.
机译：烟粉虱（Thrips tabaci）传播的鸢尾黄斑病毒（IYSV）是世界上许多洋葱种植地区鳞茎和种洋葱作物的重要经济病毒病原体。在非洲，《留尼汪岛》和《南非》已有报道。 2009年9月，在肯尼亚内罗毕种植的洋葱和韭菜上观察到疑似由IYSV引起的症状。症状包括纺锤形，稻草色，不规则的褪绿病病斑，叶片上偶有绿色小岛。用IYSV专用的Agdia Flash Kit确认了病毒的存在。随后，对肯尼亚的Makueni（2010年1月）和Mwea（2010年8月）和乌干达的Kasese（2010年1月）和Rwimi（2010年1月）洋葱生产区的小农户进行了调查。这些地区的疾病发生率在27％至72％之间。从两个位置收集的显示出IYSV感染症状的洋葱叶通过含IYSV特异性抗血清的双抗体夹心ELISA检测为病毒阳性。通过使用引物的逆转录-PCR证实了这一点。这被认为是肯尼亚和乌干达IYSV感染洋葱的第一份报告。

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