首页> 外文期刊>Plant Disease >Molecular Characterization of the sdhB Gene and Baseline Sensitivity to Penthiopyrad, Fluopyram, and Benzovindiflupyr in Venturis inaequalis
【24h】

Molecular Characterization of the sdhB Gene and Baseline Sensitivity to Penthiopyrad, Fluopyram, and Benzovindiflupyr in Venturis inaequalis

机译:sdhB基因的分子表征以及对不定芽菜中戊硫吡喃,氟吡喃和苯并文二氟吡喃的基线敏感性

获取原文
获取原文并翻译 | 示例
           

摘要

The succinate dehydrogenase inhibiting (SDHI) fungicides are a class of single-site fungicides that are increasingly important in the management of Venturia inaequalis. In this study, the baseline sensitivity of V. inaequalis to penthiopyrad, fluopyram, and benzovindiflupyr was investigated. In all, 35 to 70 isolates with no prior exposure to single-site fungicides were used to determine the effective concentration at which growth was inhibited by 50% (EC50). Mean EC50 values for the conidial germ tube growth stage for penthiopyrad, fluopyram, and benzovindiflupyr were 0.086, 0.176, and 0.0016 u-g ml-1, respectively. Linear correlation analysis revealed a significant and positive correlation between fluopyram and penthiopyrad (P S 0.0001, r = 0.66)and fluopyram and benzovindiflupyr (P = 0.0014, r = 0.52). Baseline sensitivities of V. inaequalis during the mycelial growth stagewere also determined for fluopyram and benzovindiflupyr. EC50 values were higher for fluopyram and benzovindiflupyr during this stage compared with the conidial germ tube growth stage, with means of 0.043 and 2.02 u-g ml"1, respectively. In addition, thesdhB gene was characterized for three isolates of V. inaequalis collected from a research, baseline, and commercial orchard population. No common mutation sites associated with SDHI resistance in other phytopathogenic fungi were discovered in these isolates or isolates that were recovered following field applications of SDHI fungicides. The results of this study suggest that SDHI fungicides have a high level of activity during the conidial germ tube elongation stage in V. inaequalis and provide a basisfor phenotypic and genotypic monitoring of shifts toward resistance of V. inaequalis populations to the SDHI fungicide class.
机译:琥珀酸脱氢酶抑制(SDHI)杀菌剂是一类单点杀菌剂,在不等Venturia的处理中越来越重要。在这项研究中,调查了不等纳弧菌对戊硫吡rad,氟吡草胺和苯并乙二氟吡草的基线敏感性。总共使用了35到70个事先未接触过单点杀真菌剂的分离株来确定有效浓度,在该浓度下生长被抑制50%(EC50)。五硫吡喃酮,氟吡草胺和苯并乙二氟吡啶在分生孢子胚管生长阶段的平均EC50值分别为0.086、0.176和0.0016 u-g ml-1。线性相关分析表明,氟吡草胺和戊硫吡rad(PS 0.0001,r = 0.66)与氟吡草胺和苯并二氟吡喃(P = 0.0014,r = 0.52)之间存在显着正相关。还测定了氟吡虫啉和苯并双氟吡虫在菌丝体生长阶段不等纳弧菌的基线敏感性。与分生孢子胚管生长阶段相比,该阶段氟吡虫草和苯并文二氟草醚的EC50值更高,分别为0.043和2.02 ug ml“ 1。此外,sdhB基因的特征是从三株分离到的V. inaequalis分离得到研究,基线和商业果园种群中,在这些菌株或在田间施用SDHI杀菌剂后回收的菌株中未发现与其他植物病原真菌具有SDHI抗性相关的常见突变位点。不等尖孢子菌在分生孢子生殖管延长阶段的活性水平,为监测不等尖毛孢菌种群对SDHI杀真菌剂的抗性转变提供了表型和基因型基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号