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First Report of Curvularia Leaf Blight on Curcuma wenyujin Caused by Curvularia clavata in China

机译:中国弯刀引起的温榆金弯孢叶枯病的首次报道

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Curcuma wenyujin Y.H. Chen & C. Ling is a traditional Chinese medicinal herb in the Zingiberaceae family. Commonly known as Wen yujin, the root is widely used for alleviating pain and protecting the liver. A severe leaf blight disease was observed in three C. wenyujin farms in Hainan Province of China in October 2010. The obvious symptoms of leaf blight, yellow to brown irregular lesions (1 to 20 cm) on C. wenyujin, usually began at the tips of leaves and the main veins. This disease, especially severe from August to October, caused heavy damage and 100% of mature plants (10 months old) in farms were infected. The disease was most severe when continuous cropping was performed and showed slight improvement when rotation was adopted. Farmers usually sprayed carbendazim (50% WP) and thiophanate-methyl (70% WP) to control this disease, but these treatments were not effective. To isolate the causal pathogen, diseased plants were collected in October 2010 from a field of the Hainan Branch Institute of Medicinal Plant Development in Hainan Province. Lesion tissue was removed from the border between symptomatic and healthy tissue, surface sterilized in 75% ethanol for 1 min, washed in three changes of sterile distilled water, transferred to potato dextrose agar (PDA) plates, and incubated at 28°C for 7 days.
机译:姜黄温榆金Y.H. Chen&C. Ling是姜科的传统中草药。根通常被称为温玉金,其根被广泛用于缓解疼痛和保护肝脏。 2010年10月,在中国海南省的3个温榆金鱼养殖场中观察到了严重的叶枯病病。温榆金鱼的叶枯病明显症状为黄色至棕色不规则病变(1至20 cm),通常始于尖端叶子和主脉。这种病,特别是从8月到10月的严重病害,造成了严重破坏,农场中100%成熟的植物(10个月大)被感染。连作时该病最严重,采用轮作时病情略有改善。农民通常喷洒多菌灵(50%WP)和甲基托布津(70%WP)来控制该病,但这些治疗方法无效。为了分离病原病原体,于2010年10月从海南省海南省药用植物发展研究所的田间采集了病株。从有症状和健康组织之间的边界去除病变组织,在75%的乙醇中表面灭菌1分钟,用三份无菌蒸馏水洗涤,转移至马铃薯葡萄糖琼脂(PDA)平板中,并在28°C下孵育7天。

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