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首页> 外文期刊>Plant Disease >Characterization of Diaporthe australafricana and Diaporthe spp. Associated with Stem Canker of Blueberry in Chile
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Characterization of Diaporthe australafricana and Diaporthe spp. Associated with Stem Canker of Blueberry in Chile

机译：南方飞虱和Diaporthe spp的特征。与智利的蓝莓干溃疡有关

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Stem canker and dieback are important factors that limit the longevity and reduce the yield of blueberry (Vaccinium spp.) in Chile. In this study, species of Diaporthe associated with blueberry were isolated and identified. The internal transcribed spacer (ITS) regions of ribosomal DNA of 30 isolates and the translation elongation factor 1-alpha (EF1-alpha) of 14 isolates were sequenced, analyzed, and compared with their morphological and pathological characteristics. The molecular analysis of ITS sequences by alignment with those of ex-type strains deposited in GenBank and morphological characteristics allowed the identification of Diaporthe ambigua, D. australafricana, D. neotheicola, D. passiflorae, and Diaporthe sp. 1. However, morphology alone was insufficient to identify these species. The combined analysis of ITS and EF1-alpha gene sequences grouped the Chilean blueberry isolates in the same five groups obtained in the ITS analysis. Pathogenicity tests conducted with attached and detached blueberry shoots (<1 year old) and stems (1 to 2 years old) confirmed that isolates of these Diaporthe spp. were pathogenic. The symptoms were reproducible and consisted of necrotic reddish-brown cankers on blueberry shoots and stems. These isolates were capable of infecting blueberry fruit, causing a soft decay, suggesting that they were tissue nonspecific and were also pathogenic on shoots of apple, grapevine, and pear. D. australafricana was the most frequently isolated species and D. ambigua, D. australafricana, and D. passiflorae were highly virulent in shoots, stems, and fruit of blueberry. This study showed that at least four species of Diaporthe are primary pathogens, capable of causing stem canker symptoms on blueberry, and this is the first report of D. ambigua, D. neotheicola, and D. passiflorae attacking this host.
机译：茎萎缩和枯萎是限制智利寿命并降低蓝莓（Vaccinium spp。）产量的重要因素。在这项研究中，与蓝莓有关的Diaporthe物种被分离和鉴定。对30个分离株的核糖体DNA的内部转录间隔区（ITS）区域和14个分离株的翻译延伸因子1-alpha（EF1-alpha）进行测序，分析，并将它们的形态和病理特征进行比较。通过与在GenBank中存放的前型菌株的序列进行比对和结构特征对ITS序列进行分子分析，从而鉴定出Diaporthe ambigua，D。australafricana，D。neotheicola，D.passiflorae和Diaporthe sp.。 1.但是，仅靠形态就不足以识别这些物种。 ITS和EF1-alpha基因序列的组合分析将智利蓝莓分离株分为ITS分析中获得的相同五个组。对附着和分离的蓝莓芽（<1岁）和茎（1-2岁）进行的致病性测试证实了这些Diaporthe spp的分离株。是致病的。症状是可重现的，由蓝莓芽和茎上的坏死性红褐色溃疡组成。这些分离株能够感染蓝莓果实，引起软腐烂，表明它们是组织非特异性的，并且对苹果，葡萄和梨的芽也有致病性。 D. australafricana是最常分离的物种，D。ambigua，D。australafricana和D. passiflorae在蓝莓的芽，茎和果实中具有高毒力。这项研究表明，至少有四种Diaporthe是主要病原体，能够在蓝莓上引起茎萎缩症状，这是D. ambigua，D。neotheicola和D.passiflorae攻击该寄主的第一份报道。

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