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首页> 外文期刊>Plant Disease >Genetic analysis of fungicide-resistant Sclerotinia homoeocarpa isolates from Tennessee and northern Mississippi.
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Genetic analysis of fungicide-resistant Sclerotinia homoeocarpa isolates from Tennessee and northern Mississippi.

机译：田纳西州和密西西比州北部的抗杀菌剂高果核盘菌菌株的遗传分析。

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Sclerotinia homoeocarpa is the causal agent of dollar spot disease that reduces the uniformity and aesthetic value of golf putting greens. Fungicide-resistant isolates of S. homoeocarpa were collected from putting greens at 10 locations across Tennessee and northern Mississippi. Genetic diversity among the 60 isolates was investigated using vegetative compatibility, conserved gene sequences, and amplified fragment length polymorphism (AFLP). Six tester strains were paired with Tennessee and northern Mississippi isolates on potato dextrose agar. Some of the 60 isolates were delineated into vegetative compatibility groups, but fungicide resistance could not be associated with a particular vegetative compatibility group. Genetic similarities of isolates at the vegetative compatibility level could be attributed to founder effects. Sequencing the regions of CAD, EF1- alpha , beta -tubulin, and internal transcribed spacers revealed 100% homology among isolates. Capillary gel electrophoresis and analysis of AFLP fragments indicated 86 to 100% similarity between the isolates. Vegetative compatibility and molecular data indicate that the populations of the pathogen are clonal. Isolates did not cluster according to fungicide resistance during unweighted pair group with arithmetic means analysis, but did appear to cluster according to vegetative compatibility group and location. Although associations could not be made between molecular markers and fungicide resistance, links between vegetative compatibility and AFLP markers may provide a foundation from which other studies could be performed.
机译：核果核果菌是美元斑病的病原体，会降低高尔夫球果岭的均匀性和美学价值。在田纳西州和密西西比州北部的10个地点的果岭中收集了抗果蝇链霉菌的分离株。使用营养相容性，保守基因序列和扩增片段长度多态性（AFLP）研究了60个分离株之间的遗传多样性。在马铃薯葡萄糖琼脂上将六种测试菌株与田纳西州和北密西西比分离株配对。在60个分离物中，有一些被划分为营养相容性组，但抗药性不能与特定的营养相容性组相关。分离株在营养相容性水平上的遗传相似性可归因于创始人效应。对CAD，EF1-α，β-微管蛋白和内部转录间隔区的序列进行测序显示，分离株之间具有100％的同源性。毛细管凝胶电泳和AFLP片段分析表明分离株之间有86％到100％的相似性。营养相容性和分子数据表明该病原体是克隆的。通过算术平均值分析，在未加权对组中，分离物并未根据杀菌剂的抗性进行聚类，但根据营养相容性组和位置而出现了聚类。尽管无法在分子标记和抗真菌剂之间建立联系，但营养相容性与AFLP标记之间的联系可能为开展其他研究提供基础。

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