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首页> 外文期刊>Plant physiology >PHYSICAL ASSOCIATION OF STARCH BIOSYNTHETIC ENZYMES WITH STARCH GRANULES OF MAIZE ENDOSPERM - GRANULE-ASSOCIATED FORMS OF STARCH SYNTHASE I AND STARCH BRANCHING ENZYME II
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PHYSICAL ASSOCIATION OF STARCH BIOSYNTHETIC ENZYMES WITH STARCH GRANULES OF MAIZE ENDOSPERM - GRANULE-ASSOCIATED FORMS OF STARCH SYNTHASE I AND STARCH BRANCHING ENZYME II

机译：淀粉内胚乳淀粉颗粒的淀粉生物合成酶的物理结合-淀粉合成酶I和淀粉分支酶II的颗粒伴随形式。

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Antibodies were used to probe the degree of association of starch biosynthetic enzymes with starch granules isolated from maize (Zea mays) endosperm. Graded washings of the starch granule, followed by release of polypeptides by gelatinization in 2% sodium dodecyl sulfate, enables distinction between strongly and loosely adherent proteins. Mild aqueous washing of granules resulted in near-complete solubilization of ADP-glucose pyrophosphorylase, indicating that little, if any, ADP-glucose pyrophosphorylase is granule associated. In contrast, all of the waxy protein plus significant levels of starch synthase I and starch branching enzyme II (BEII) remained granule associated. Stringent washings using protease and detergent demonstrated that the waxy protein, more than 85% total endosperm starch synthase I protein, and more than 45% of BEII protein were strongly associated with starch granules. Rates of polypeptide accumulation within starch granules remained constant during endosperm development. Soluble and granule-derived forms of BEII yielded identical peptide maps and overlapping tryptic fragments closely aligned with deduced amino acid sequences from BEII cDNA clones. These observations provide direct evidence that BEII exits as both soluble and granule-associated entities. We conclude that each of the known starch biosynthetic enzymes in maize endosperm exhibits a differential propensity to associate with, or to become irreversibly entrapped within, the starch granule. [References: 35]
机译：使用抗体来探测淀粉生物合成酶与分离自玉米（Zea mays）胚乳的淀粉颗粒的缔合程度。淀粉颗粒的分级洗涤，然后通过在2％十二烷基硫酸钠中进行糊化来释放多肽，可以区分强粘附和松散粘附的蛋白质。轻度水洗颗粒会使ADP-葡萄糖焦磷酸化酶几乎完全溶解，表明几乎没有（如果有的话）ADP-葡萄糖焦磷酸化酶与颗粒相关。相反，所有蜡质蛋白加上显着水平的淀粉合酶I和淀粉分支酶II（BEII）仍与颗粒相关。使用蛋白酶和去污剂的严格洗涤表明，蜡质蛋白，总胚乳淀粉合酶I蛋白的85％以上和BEII蛋白的45％以上与淀粉颗粒紧密相关。在胚乳发育过程中，淀粉颗粒内多肽积累的速率保持恒定。可溶性和颗粒形式的BEII产生相同的肽图，并且重叠的胰蛋白酶片段与从BEII cDNA克隆推导的氨基酸序列紧密对齐。这些观察结果提供了直接证据，表明BEII以可溶性和颗粒相关实体的形式存在。我们得出的结论是，玉米胚乳中每种已知的淀粉生物合成酶均表现出与淀粉颗粒缔合或不可逆地包埋在淀粉颗粒中的不同倾向。 [参考：35]

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