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首页> 外文期刊>Plant physiology >Proteomic analysis of chloroplast-to-chromoplast transition in tomato reveals metabolic shifts coupled with disrupted thylakoid biogenesis machinery and elevated energy-production components
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Proteomic analysis of chloroplast-to-chromoplast transition in tomato reveals metabolic shifts coupled with disrupted thylakoid biogenesis machinery and elevated energy-production components

机译：番茄中叶绿体向染色体的转变的蛋白质组学分析揭示了代谢变化以及类囊体生物发生机制的破坏和能量产生成分的升高

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A comparative proteomic approach was performed to identify differentially expressed proteins in plastids at three stages of tomato (Solanum lycopersicum) fruit ripening (mature-green, breaker, red). Stringent curation and processing of the data from three independent replicates identified 1,932 proteins among which 1,529 were quantified by spectral counting. The quantification procedures have been subsequently validated by immunoblot analysis of six proteins representative of distinct metabolic or regulatory pathways. Among the main features of the chloroplast-to-chromoplast transition revealed by the study, chromoplastogenesis appears to be associated with major metabolic shifts: (1) strong decrease in abundance of proteins of light reactions (photosynthesis, Calvin cycle, photorespiration) and carbohydrate metabolism (starch synthesis/degradation), mostly between breaker and red stages and (2) increase in terpenoid biosynthesis (including carotenoids) and stress-response proteins (ascorbate-glutathione cycle, abiotic stress, redox, heat shock). These metabolic shifts are preceded by the accumulation of plastid-encoded acetyl Coenzyme A carboxylase D proteins accounting for the generation of a storage matrix that will accumulate carotenoids. Of particular note is the high abundance of proteins involved in providing energy and in metabolites import. Structural differentiation of the chromoplast is characterized by a sharp and continuous decrease of thylakoid proteins whereas envelope and stroma proteins remain remarkably stable. This is coincident with the disruption of the machinery for thylakoids and photosystem biogenesis (vesicular trafficking, provision of material for thylakoid biosynthesis, photosystems assembly) and the loss of the plastid division machinery. Altogether, the data provide new insights on the chromoplast differentiation process while enriching our knowledge of the plant plastid proteome.
机译：进行了比较蛋白质组学方法，以鉴定番茄（Solanum lycopersicum）果实成熟的三个阶段（成熟的绿色，破碎的红色）中质体中差异表达的蛋白质。严格管理和处理来自三个独立重复样品的数据，鉴定出1,932种蛋白质，其中1,529种通过光谱计数进行了定量。定量程序随后已通过对代表独特代谢或调节途径的六种蛋白质进行免疫印迹分析进行了验证。在这项研究揭示的叶绿体到色体的过渡的主要特征中，色体发生似乎与主要的代谢变化有关：（1）光反应（光合作用，卡尔文循环，光呼吸）和碳水化合物代谢的蛋白质丰度大大降低（淀粉的合成/降解），主要是在破乳阶段和红色阶段之间;（2）萜类生物合成（包括类胡萝卜素）和应激反应蛋白（抗坏血酸-谷胱甘肽循环，非生物胁迫，氧化还原，热休克）增加。在这些代谢转变之前，质体编码的乙酰辅酶A羧化酶D蛋白的积累，说明了将积累类胡萝卜素的存储基质的产生。特别值得注意的是，参与提供能量和代谢产物输入的蛋白质含量很高。原生质体的结构分化的特征是类囊体蛋白急剧而连续地减少，而包膜和基质蛋白保持非常稳定。这与类囊体机制和光系统生物发生（囊泡运输，类囊体生物合成所需材料的提供，光系统组装）的破坏以及质体分裂机制的丧失相吻合。总而言之，这些数据在丰富我们对植物质体蛋白质组的知识的同时，为染色体细胞分化过程提供了新的见解。

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