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首页> 外文期刊>Plant physiology >The significance of protein maturation by plastidic type I signal peptidase 1 for thylakoid development in Arabidopsis chloroplasts
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The significance of protein maturation by plastidic type I signal peptidase 1 for thylakoid development in Arabidopsis chloroplasts

机译：I型质体信号肽酶1的蛋白质成熟对于拟南芥叶绿体类囊体发育的意义

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摘要

Thylakoids are the chloroplast internal membrane systems that house light-harvesting and electron transport reactions. Despite the important functions and well-studied constituents of thylakoids, the molecular mechanism of their development remains largely elusive. A recent genetic study has demonstrated that plastidic type I signal peptidase 1 (Plsp1) is vital for proper thylakoid development in Arabidopsis (Arabidopsis thaliana) chloroplasts. Plsp1 was also shown to be necessary for processing of an envelope protein, Toc75, and a thylakoid lumenal protein, OE33; however, the relevance of the protein maturation in both of the two distinct subcompartments for proper chloroplast development remained unknown. Here, we conducted an extensive analysis of the plsp1-null mutant to address the significance of lumenal protein maturation in thylakoid development. Plastids that lack Plsp1 were found to accumulate vesicles of variable sizes in the stroma. Analyses of the mutant plastids revealed that the lack of Plsp1 causes a reduction in accumulation of thylakoid proteins and that Plsp1 is involved in maturation of two additional lumenal proteins, OE23 and plastocyanin. Further immunoblotting and electron microscopy immunolocalization studies showed that OE33 associates with the stromal vesicles of the mutant plastids. Finally, we used a genetic complementation system to demonstrate that accumulation of improperly processed forms of Toc75 in the plastid envelope does not disrupt normal plant development. These results suggest that proper maturation of lumenal proteins may be a key process for correct assembly of thylakoids.
机译：类囊体是容纳光收集和电子传输反应的叶绿体内部膜系统。尽管类囊体具有重要的功能和经过充分研究的成分，但其​​发展的分子机制仍然难以捉摸。最近的一项遗传研究表明，塑性I型信号肽酶1（Plsp1）对于拟南芥（Arabidopsis thaliana）叶绿体中类囊体正常发育至关重要。还显示Plsp1对于加工包膜蛋白Toc75和类囊体腔蛋白OE33是必需的;然而，两个不同的小室中的蛋白质成熟与叶绿体正常发育的相关性仍然未知。在这里，我们对plsp1-null突变体进行了广泛的分析，以解决类囊体发育中管腔蛋白成熟的重要性。缺乏Plsp1的质体被发现在基质中积聚可变大小的囊泡。突变体的分析表明，Plsp1的缺乏会导致类囊体蛋白的积累减少，并且Plsp1参与了另外两种腔蛋白OE23和质体蓝蛋白的成熟。进一步的免疫印迹和电子显微镜免疫定位研究表明，OE33与突变质体的基质囊泡相关。最后，我们使用了一种遗传互补系统来证明在质体包膜中不正确加工的Toc75形式的积累不会破坏正常的植物发育。这些结果表明，腔蛋白的正确成熟可能是类囊体正确组装的关键过程。

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