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首页> 外文期刊>Plant Physiology and Biochemistry >Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection
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Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection

机译：转基因烟草植物中棉花亲环蛋白基因（GhCyp1）的过表达赋予了对盐胁迫和丁香假单胞菌pv的双重耐受性。烟粉虱感染

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摘要

The full-length cDNA of a cyclophilin-like gene was cloned from Gossypium hirsutum using rapid amplification of cDNA ends and was designated as GhCyp1, a member of the immunophilin protein family. GhCyp1 expression level was higher in roots and stems than in other tissues of cotton, as determined by real-time reverse transcription polymerase chain reaction (RT-PCR). To characterize the GhCyp1 gene, tobacco (Nicotiana tabacum) was transformed via Agrobacterium tumefaciens with a vector to express the gene under the control of a strong constitutive promoter, CaMV35S (Cauliflower Mosaic Virus). Based on analyses of tolerance to salinity stress and Pseudomonas syringae pv. tabaci (Pst) infection, the overexpression of GhCyp1 in transgenic plants conferred higher tolerance to salt stress and Pst infection compared with control plants. Therefore, we suggest that GhCyp1 may be a suitable candidate gene to produce transgenic plants with tolerance to abiotic and biotic stresses.
机译：使用快速扩增cDNA末端的方法，从陆地棉中克隆了亲环蛋白样基因的全长cDNA，并将其命名为GhCyp1，这是亲免蛋白家族的成员。实时逆转录聚合酶链反应（RT-PCR）确定，GhCyp1在根和茎中的表达水平高于棉花的其他组织。为了表征GhCyp1基因，通过根癌农杆菌用载体转化烟草（烟草），以在强组成型启动子CaMV35S（花椰菜花叶病毒）的控制下表达该基因。基于对盐分胁迫和丁香假单胞菌PV的耐受性分析。烟粉虱（Pst）感染，与对照植物相比，转基因植物中GhCyp1的过度表达赋予了更高的耐盐胁迫和Pst感染的能力。因此，我们建议GhCyp1可能是合适的候选基因，以产生对非生物和生物胁迫具有耐受性的转基因植物。

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