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首页> 外文期刊>Plant Physiology and Biochemistry >Involvement of phospholipase D and NADPH-oxidase in salicylic acid signaling cascade
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Involvement of phospholipase D and NADPH-oxidase in salicylic acid signaling cascade

机译：磷脂酶D和NADPH氧化酶参与水杨酸信号级联反应

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摘要

Salicylic acid is associated with the primary defense responses to biotic stress and formation of systemic acquired resistance. However, molecular mechanisms of early cell reactions to phytohormone application are currently undisclosed. The present study investigates the participation of phospholipase D and NADPH-oxidase in salicylic acid signal transduction cascade.The activation of lipid signaling enzymes within 15 min of salicylic acid application was shown in Arabidopsis thaliana plants by measuring the phosphatidic acid accumulation. Adding of primary alcohol (1-butanol) to the incubation medium led to phosphatidylbutanol accumulation as a result of phospholipase D (PLD) action in wild-type and NADPH-oxidase RbohD deficient plants. Salicylic acid induced rapid increase in NADPH-oxidase activity in histochemical assay with nitroblue tetrazolium but the reaction was not observed in presence of 1-butanol and NADPH-oxidase inhibitor diphenylene iodide (DPI). The further physiological effect of salicylic acid and inhibitory analysis of the signaling cascade were made in the guard cell model. Stomatal closure induced by salicylic acid was inhibited by 1-butanol and DPI treatment. rbohD transgenic plants showed impaired stomatal reaction upon phytohormone effect, while the reaction to H_2O_2 did not differ from that of wild-type plants. Thus a key role of NADPH-oxidase D-isoform in the process of stomatal closure in response to salicylic acid has been postulated. It has enabled to predict a cascade implication of PLD and NADPH oxidase to salicylic acid signaling pathway.
机译：水杨酸与对生物胁迫的初步防御反应和系统获得性抗性的形成有关。然而，目前尚未公开早期细胞对植物激素的反应的分子机制。本研究调查了磷脂酶D和NADPH-氧化酶在水杨酸信号转导级联反应中的参与作用。通过测量磷脂酸的积累，拟南芥植物在水杨酸施用15分钟内脂质信号酶的激活情况。在野生型和NADPH-氧化酶RbohD缺陷型植物中，由于磷脂酶D（PLD）的作用，向培养液中添加伯醇（1-丁醇）导致磷脂酰丁醇积聚。水杨酸在使用硝基蓝四唑的组织化学分析中诱导NADPH-氧化酶活性快速增加，但在1-丁醇和NADPH-氧化酶抑制剂二苯碘化物（DPI）的存在下未观察到反应。水杨酸的进一步生理作用和对信号级联反应的抑制分析在保卫细胞模型中进行。水杨酸诱导的气孔关闭被1-丁醇和DPI处理抑制。 rbohD转基因植物受植物激素作用显示出气孔反应受损，而对H_2O_2的反应与野生型植物无差异。因此，已经假定NADPH-氧化酶D-同工型在响应水杨酸的气孔关闭过程中起关键作用。它已经能够预测PLD和NADPH氧化酶对水杨酸信号通路的级联影响。

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