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首页> 外文期刊>Plant Physiology and Biochemistry >Globular body production, their anatomy, DNase gel analysis and NDP kinase activity in root tips of Poncirus trifoliata L.
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Globular body production, their anatomy, DNase gel analysis and NDP kinase activity in root tips of Poncirus trifoliata L.

机译：三叶半夏的根尖中的球状体产生，其解剖结构，DNase凝胶分析和NDP激酶活性。

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摘要

Green globular bodies were developed from Poncirus trifoliata L. root tip explants as a response to addition in the substrate of different growth regulators. From the globular bodies, shoots initiated and grew. Median section of the globular bodies reveals that they are composed of parenchyma cells and originate from the pericycle. The activity of DNases during shoot formation from globular bodies was influenced by the type and concentration of plant growth regulators that were added in the nutrient substrate. Peptide bands formation was also influenced by the increase of BA concentration. Consequently, BA, NAA and IAA combination influenced 5'-triphosphonucleosides (NTPs) appearance and activity in the presence of metal. Peptide bands resulted from the electrophoretic analysis of endogenous protein phosphorylation, proved to be catalytic subunits of NDP kinases, as they all phosphorylate diphosphonucleosides. The enzymes DNases and NDP kinases could be used as a scientific tool for the study of shoot formation from P.trifoliata L. green globular bodies.
机译：绿色的球状体是从Poncirus trifoliata L.根尖外植体发育而来的，作为对不同生长调节剂添加到基质中的响应。从球状体开始萌芽并生长。球状体的中段显示它们由薄壁组织组成，起源于周周。从球状体形成芽的过程中，DNases的活性受营养基质中添加的植物生长调节剂的类型和浓度的影响。肽带的形成也受到BA浓度增加的影响。因此，BA，NAA和IAA的组合会在金属存在下影响5'-三磷酸核苷（NTP）的外观和活性。电泳分析内源蛋白磷酸化产生的肽带被证明是NDP激酶的催化亚基，因为它们都磷酸化了二磷酸核苷。 DNases和NDP激酶可作为研究P.trifoliata L.绿色球状体芽形成的科学工具。

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