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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Dwarf (di)haploid pito mutants obtained from a tetraploid potato cultivar (Solanum tuberosum subsp. tuberosum) via anther culture are defective in gibberellin biosynthesis
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Dwarf (di)haploid pito mutants obtained from a tetraploid potato cultivar (Solanum tuberosum subsp. tuberosum) via anther culture are defective in gibberellin biosynthesis

机译：通过花药培养从四倍体马铃薯栽培品种（马铃薯）得到的矮（二）单倍体pito突变体在赤霉素生物合成中存在缺陷

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摘要

Nine dwarf (di)haploid lines (2n = 2 x = 24) were obtained from the tetraploid (2n = 4 x = 48), long day-adapted potato cultivar 'Pito' (Solanum tuberosum subsp. tuberosum) through anther culture. They grew slowly, had very short internodes, compact and ball-shaped appearance, and dark green leaves. Dwarfism was due to a recessive gene, designated pito. Endogenous gibberellin contents were measured in the leaves of dwarf and wild-type lines by gas chromatography linked to mass spectrometry (GC-MS). High amounts of GA inferior 1 inferior 9, GA inferior 2 inferior 0, GA inferior 2 inferior 9, GA inferior 1, and GA inferior 8 were detected in the wild-type plants, which indicated that the early 13-hydroxylation pathway was predominantly used for GA biosynthesis in S. t. subsp. tuberosum. Also GA inferior 5 inferior 3, GA inferior 1 inferior 5 and GA inferior 9 were detected but not quantified. Very low endogenous amounts of all analysed GAs were detected in the pito mutants, indicating a block at an early part of the GA biosynthesis pathway. The dwarf lines strongly and quickly responded to the exogenous application of low amounts (79 nM) of bioactive GA (GA inferior 3), which restored normal growth and confirmed that the pito dwarfs were synthesis mutants and not GA response mutants.
机译：通过花药培养从四倍体（2n = 4 x = 48），长日适应性马铃薯品种'Pito'（马铃薯ola变亚种）中获得了九个矮（二）单倍体系（2n = 2 x = 24）。它们生长缓慢，节间非常短，外观紧凑而球形，还有深绿色的叶子。侏儒症是由于隐性基因pito引起的。通过与质谱联用的气相色谱法（GC-MS）测量矮化和野生型品系叶片中的内源赤霉素含量。在野生型植物中检测到大量的GA下1，GA 9，GA 2低于0，GA下2，GA 9以及GA下8，这表明早期使用了13-羟基化途径。 S. t。中的GA生物合成亚种马铃薯还检测到GA下5，GA下1，GA 5下和GA下9。在pito突变体中检测到的所有内源GA的内源性含量极低，这表明GA生物合成途径的早期部分受阻。矮株系对少量生物活性GA（GA下3）的外源施用（79 nM）有强烈而迅速的反应，这恢复了正常的生长并证实了皮托矮株是合成突变体而不是GA反应突变体。

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