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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Hydroxyproline-rich glycopeptide signals in potato elicit signalling associated with defense against insects and pathogens.
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Hydroxyproline-rich glycopeptide signals in potato elicit signalling associated with defense against insects and pathogens.

机译:马铃薯中富含羟脯氨酸的糖肽信号引发与昆虫和病原体防御相关的信号。

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摘要

HypSys peptides are 18-20 amino acids glycopeptide defense signal first discovered in tobacco and tomato that activate expression of defensive genes against insect-herbivores. Discovery of their orthologs in other Solanaceaous and nonsolanaceous plants demonstrated their possible ubiquitous nature and species specific functional diversity. In our continued search to establish the paradigm of defense signalling by HypSys peptides, we isolated a cDNA from potato leaves encoding putative analogs of tomato HypSys peptides flanked by conserved proteolytic cleavage sites. The gene encoding the cDNA was a member of a gene family in the tetraploid genome of potato and its expression was transcriptionally activated by wounding and methyl jasmonate. The deduced precursor protein contained a leader peptidase splice site and three putative HypSys peptides with conserved N- and C-termini along with central proline-rich motifs. In defense signalling, the three HypSys peptides elicit H2O2 generation in vivo and activate several antioxidant defensive enzymes in young potato leaves. Similar to potato systemin, the HypSys peptides activate the expression of octadecanoid pathway genes and protease inhibitors for insect defense. In addition, the HypSys peptides also activate the essential genes of the innate pathogen defense response in young potato leaves, acting as common elicitors of signalling associated with anti-herbivore and anti-pathogen defense in potato.
机译:HypSys肽是一种在烟草和番茄中最先发现的18-20个氨基酸的糖肽防御信号,它能激活针对昆虫除草剂的防御基因的表达。在其他茄科和非茄科植物中发现它们的直系同源物证明了它们可能普遍存在的性质和物种特异性的功能多样性。在我们继续寻找通过HypSys肽建立防御信号传导范式的过程中,我们从马铃薯叶中分离了cDNA,该cDNA编码了番茄HypSys肽的假定类似物,两侧是保守的蛋白水解切割位点。编码cDNA的基因是马铃薯四倍体基因组中一个基因家族的成员,其表达通过创伤和茉莉酸甲酯被转录激活。推导的前体蛋白包含一个前导肽酶剪接位点和三个假定的HypSys肽,具有保守的N和C末端以及富含脯氨酸的基序。在防御信号传导中,这三种HypSys肽在体内诱导H 2 O 2 生成并激活年轻马铃薯叶片中的几种抗氧化防御酶。与马铃薯systemin相似,HypSys肽激活十八烷类通路基因和蛋白酶抑制剂的表达,以防御昆虫。另外,HypSys肽还激活年轻马铃薯叶片中先天病原体防御反应的必需基因,充当与马铃薯中抗草食动物和抗病原体防御相关的信号的常见引发剂。

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