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首页> 外文期刊>Biomaterials >Fibrinogen surface distribution correlates to platelet adhesion pattern on fluorinated surface-modified polyetherurethane
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Fibrinogen surface distribution correlates to platelet adhesion pattern on fluorinated surface-modified polyetherurethane

机译：纤维蛋白原表面分布与氟化表面改性聚醚氨酯上的血小板粘附模式相关

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摘要

In previous work, it had been shown that platelet adhesion could be reduced by fluorinating surfaces with oligomeric fluoropolymers, referred to as surface-modifying macromolecules (SMMs). In the current study, two in vitro blood-contacting experiments were carried out on a polyetherurethane modified with three different SMMs in order to determine if altered platelet adhesion levels could be related to the pattern of adsorbed protein and more specifically to the manner in which fibrinogen (Fg) distribution occurs at the surface. In the first experiment, the materials were placed in whole human blood and the adherent platelets were viewed with high-resolution scanning electron microscopy (SEM). In a second experiment, the materials were incubated with human plasma with the absence of platelets. The plasma contained 5% fluorescent-Fg. The materials were then viewed with a fluorescence microscope and images were collected to define the distribution of high-density fluorescent-Fg areas. The SEM and fluorescent-Fg images were imported to Image Pro Plus (TM) imaging software to measure the area, length and circularity and a bivariate correlation test was conducted between the two sets of data. For area and length morphology parameters, there were high and significant correlations (r > 0.9, p < 0.05) between the platelets and Fg aggregates. The data suggest that the Fg distribution may serve as a predictor of platelet morphology/activation and provides insight into the non-thrombogenic character of biomaterials containing the fluorinated SMMs. (c) 2005 Elsevier Ltd. All rights reserved.
机译：在以前的工作中，已经表明可以通过用低聚含氟聚合物（称为表面改性大分子（SMM））氟化表面来减少血小板粘附。在当前的研究中，对用三种不同的SMM改性的聚醚氨酯进行了两次体外血液接触实验，以确定改变的血小板粘附水平是否可能与吸附蛋白的模式有关，更具体地说与纤维蛋白原的方式有关。 （Fg）分布发生在表面。在第一个实验中，将材料置于全血中，并用高分辨率扫描电子显微镜（SEM）观察粘附的血小板。在第二个实验中，将材料与人血浆在不存在血小板的情况下孵育。血浆包含5％的荧光-Fg。然后用荧光显微镜观察材料，并收集图像以定义高密度荧光Fg区域的分布。将SEM和荧光Fg图像导入Image Pro Plus（TM）成像软件以测量面积，长度和圆度，并在两组数据之间进行了双变量相关性测试。对于面积和长度形态参数，血小板和Fg聚集体之间存在高度显着的相关性（r> 0.9，p <0.05）。数据表明Fg分布可作为血小板形态/激活的预测指标，并提供对含氟化SMM的生物材料的非血栓形成特性的见解。 （c）2005 Elsevier Ltd.保留所有权利。

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