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首页> 外文期刊>Plant Molecular Biology >THE MYROSINASE GENE FAMILY IN ARABIDOPSIS THALIANA - GENE ORGANIZATION, EXPRESSION AND EVOLUTION
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THE MYROSINASE GENE FAMILY IN ARABIDOPSIS THALIANA - GENE ORGANIZATION, EXPRESSION AND EVOLUTION

机译：拟南芥中的芥子酶基因家族-基因的组织，表达和进化

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Myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1.)is in Brassicaceae species such as Brassica napus and Sinapis alba encoded by two differentially expressed gene families, MA and MB, consisting of about 4 and 10 genes, respectively. Southern blot analysis showed that Arabidopsis thaliana contains three myrosinase genes. These genes were isolated from a genomic library and two of them, TGG1 and TGG2, were sequenced. They were found to be located in an inverted mode with their 3' ends 4.4 kb apart. Their organization was highly conserved with 12 exons and 11 short introns. Comparison of nucleotide sequences of TGG1 and TGG2 exons revealed an overall 75% similarity. In contrast, the overall nucleotide sequence similarity in introns was only 42%. In intron 1 the unusual 5' splice border GC was used. Phylogenetic analyses using both distance matrix and parsimony programs suggested that the Arabidopsis genes could not be grouped with either MA or MB genes. Consequently, these two gene families arose only after Arabidopsis had diverged from the other Brassicaceae species. In situ hybridization experiments showed that TGG1 and TGG2 expressing cells are present in leaf, sepal, petal, and gynoecium. In developing seeds, a few cells reacting with the TGG1 probe, but not with the TGG2 probe, were found indicating a partly different expression of these genes. [References: 38]
机译：黑芥子酶（硫代葡萄糖苷葡糖水解酶，EC 3.2.3.1。）存在于芸苔科物种中，例如甘蓝型油菜和白芥子，由两个差异表达的基因家族MA和MB编码，分别由约4个和10个基因组成。 Southern印迹分析表明拟南芥含有三个黑芥子酶基因。从基因组文库中分离了这些基因，并对其中的两个TGG1和TGG2进行了测序。发现它们以反向模式定位，其3'末端间隔为4.4 kb。他们的组织高度保守，有12个外显子和11个短内含子。比较TGG1和TGG2外显子的核苷酸序列可发现总体相似性为75％。相反，内含子的总体核苷酸序列相似性仅为42％。内含子1中使用了不常见的5'剪接边界GC。使用距离矩阵和简约程序的系统发育分析表明拟南芥基因不能与MA或MB基因分组。因此，这两个基因家族仅在拟南芥与其他十字花科物种分离后才出现。原位杂交实验表明，表达TGG1和TGG2的细胞存在于叶片，萼片，花瓣和妇科。在发育中的种子中，发现一些细胞与TGG1探针反应，但与TGG2探针没有反应，表明这些基因的表达部分不同。 [参考：38]

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