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首页> 外文期刊>Plant Molecular Biology >A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)
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A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)

机译：一组基于GFP的细胞器标记线与基于DsRed的网关载体结合用于水稻（Oryza sativa L.）的亚细胞定位研究

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In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.
机译：在后基因组时代，开发了许多有用的工具来加速对基因功能的研究。荧光蛋白已被广泛用作蛋白质标签，用于研究植物中蛋白质的亚细胞定位。在双子叶植物中已经产生了几种荧光细胞器标记物系。然而，单子叶植物型水稻缺乏有用且可靠的荧光细胞器标记物系。在这里，我们在转基因水稻中开发了八个不同的基于GFP的细胞器标记，并创建了一套基于DsRed的网关载体以与标记线结合。两个线粒体定位的水稻抗坏血酸过氧化物酶基因与DsRed融合并成功与线粒体靶向标记物共定位，证明了该系统的实际应用。 GFP融合标记物系和DsRed融合蛋白的共定位为水稻蛋白亚细胞定位的体内或体外分析提供了便利的平台。

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