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首页> 外文期刊>Plant Pathology >Long-term survival of Acidovorax citrulli in citron melon (Citrullus lanatus var. citroides) seeds
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Long-term survival of Acidovorax citrulli in citron melon (Citrullus lanatus var. citroides) seeds

机译：柠檬酸瓜果种子在柠檬柚子中的长期存活率（Citrullus lanatus var。citroides）种子

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Long-term survival of Acidovorax citrulli in citron melon (Citrullus lanatus var. citroides) seeds was investigated. Citron melon seed lots infected with A. citrulli were generated in the field by inoculating either the pistils (stigma) or pericarps (ovary wall) of the female blossoms. Seventeen A. citrulli isolates from 14 different haplotypes belonging to two different groups (group I and II) were used for inoculation. After confirming that 100% of seed lots were infected, they were stored at 4°C and 50% RH for 7 years. After storage, the viability of A. citrulli cells from individual lots was determined by plating macerated seeds on semiselective medium as well as growing seeds for 14 days and scoring for bacterial fruit blotch symptoms. The type of A. citrulli isolate (group I or group II) used did not significantly influence bacterial survival. However, A. citrulli survival was significantly greater in seed lots generated via pistil inoculation (52·9 and 29·4%) than via pericarp inoculation (23·5 and 17·6%). Repetitive extragenic palindrome (rep)-PCR on A. citrulli isolated from citron melon seed lots after storage displayed similar fingerprinting patterns to those of the reference strains originally used for blossom inoculation, indicating that cross-contamination did not occur. The results indicate that A. citrulli may survive/overwinter in citron melon seeds for at least 7 years and bacterial survival in seed was influenced more by method of blossom inoculation than by the type of bacterial isolate.
机译：研究了酸果酸瓜在柚子（Citrullus lanatus var。citroides）种子中的长期存活。通过接种雌花的雌蕊（柱头）或果皮（子房壁），在田间产生了感染了瓜曲霉的柚子瓜种子。来自两个不同组（I和II组）的14种不同单倍型的十七种A. citrulli分离株用于接种。确认100％的种子已被感染后，将它们在4°C和50％RH下保存7年。储存后，通过将浸软的种子铺在半选择培养基上以及生长种子14天并评估细菌性果斑症状来确定单个批次的瓜曲霉细胞的生存能力。所用瓜曲霉分离物的类型（I组或II组）对细菌存活没有明显影响。但是，通过雌蕊接种产生的种子批次（52·9和29·4％）中的瓜果农杆菌的存活率显着高于果皮接种（23·5和17·6％）。贮藏后从柚子瓜种子批次中分离的柑桔曲霉的重复基因外回文（rep）-PCR显示出与最初用于开花接种的参考菌株相似的指纹图谱，表明未发生交叉污染。结果表明，瓜果曲霉可以在柚子瓜种子中存活/越冬至少7年，并且开花接种方法比细菌分离物的类型对种子中细菌存活的影响更大。

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