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首页> 外文期刊>Plant Pathology >Benzothiadiazole-primed defence responses and enhanced differential expression of defence genes in Vitis vinifera infected with biotrophic pathogens Erysiphe necator and Plasmopara viticola
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Benzothiadiazole-primed defence responses and enhanced differential expression of defence genes in Vitis vinifera infected with biotrophic pathogens Erysiphe necator and Plasmopara viticola

机译：苯并噻二唑引发的防御反应和防御基因的增强表达在感染了生物营养性病原体Erysiphe necator和Plasmopara viticola的葡萄中

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摘要

Benzothiadiazole (BTH), a salicylic acid analogue, strengthens plant defence mechanisms against a broad spectrum of pathogens. The role of pre-treatment with BTH in enhancing resistance against infection with various isolates of downy and powdery mildews (Plasmopara viticola and Erysiphe necator) was investigated in grapevine leaves. Tools were developed to better assess the defence status of the plant. In compatible interactions amongst a set of 19 genes, more than 57·2% of differentiated transcripts from P. viticola infected-leaves (Pv-infected leaves) and 90% from E. necator-infected leaves (Ew-infected leaves) were down-regulated at 24 h post-inoculation (hpi), indicating a manipulation of host responses by the pathogens. BTH treatment enhanced grapevine defences, with pathogen growdi inhibited by 61-98%, depending on the pathogen isolate. Treatment also triggered up-regulation of pathogenesis-related protein genes such as PR-1, PR-2, PR-3, PR-8 and PR-10 in Pv-infected leaves, and PR-3, PR-6 and PR-10 in En-infected leaves. Treatment with BTH also led to regulation of indole pamway transcripts; in particular, anthranilate synthase was down-regulated at 24 hpi in all infected leaves, then strongly up-regulated afterwards according to the rate of pathogen development. Quantitation of polyphenols and stilbenes showed mat pterostilbene was specifically accumulated in pre-treated leaves and associated with biological efficacy and significant increases in PR protein gene transcripts. The temporal evolution of defence-related genes in pre-treated infected leaves suggests that grapevine responses vary depending on the inter- or intra-species variability of pathogens.
机译：水杨酸类似物苯并噻二唑（BTH）增强了针对多种病原体的植物防御机制。在葡萄叶片中研究了用BTH预处理增强霜霉病和白粉病菌分离株（Plasmopara viticola和Erysiphe necator）抵抗感染的作用。开发了可以更好地评估工厂防御状态的工具。在一组19个基因之间的相容性相互作用中，来自葡萄球菌感染的叶子（受Pv感染的叶子）和大肠杆菌感染的叶子（受Ew感染的叶子）分化的转录本中超过57·2％的转录本下降了。在接种后24小时（hpi）调节-指示病原体对宿主反应的操纵。 BTH处理增强了葡萄的防御能力，病原体的生长抑制了61-98％，具体取决于病原体的分离物。处理还触发了与病程相关的蛋白质基因的上调，例如感染Pv的叶片中的PR-1，PR-2，PR-3，PR-8和PR-10，以及PR-3，PR-6和PR- 10在被感染的叶子中。 BTH的治疗还导致对吲哚pamway转录物的调节。特别是，在所有感染的叶片中，邻氨基苯甲酸合酶在24 hpi下调，然后根据病原体的生长速度强烈上调。多酚和对苯二甲酸酯的定量显示，伞形蕨类植物特别积累在预处理的叶片中，并与生物学功效和PR蛋白基因转录物显着增加有关。预先处理的受感染叶片中与防御相关的基因的时间演变表明，葡萄藤的反应根据病原体的种间或种内变异而变化。

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