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首页> 外文期刊>Plant Pathology >Baseline sensitivity of natural populations and resistance of mutants of Xanthomonas oryzae pv. oryzae to a novel bactericide, zinc thiazole
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Baseline sensitivity of natural populations and resistance of mutants of Xanthomonas oryzae pv. oryzae to a novel bactericide, zinc thiazole

机译：自然种群的基线敏感性和水稻黄单胞菌突变体的抗性。米粉对新型杀菌剂噻唑锌

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摘要

During 2009-2010, a total of 323 isolates of Xanthomonas oryzae pv. oryzae were obtained from rice with symptoms of bacterial leaf blight (BLB) in four provinces (Zhejiang, Jiangsu, Anhui and Hubei) in China. These isolates were tested for baseline sensitivity to zinc thiazole, a novel bactericide with strong antibacterial activity against Xanthomonas. The sampled pathogenic population had similar sensitivity to zinc thiazole (OT-16-8 mg L~(-1)) in all four regions and over the whole two-year study period. The baseline sensitivity was distributed as a unimodal curve with a mean ECso value of 6·79 ± 1·61 mg L~(-1) . The risk of mutation to resistance of zinc thiazole in X. oryzae pv. oryzae was further evaluated in vitro and in vivo. Twelve zinc thiazole-resistant mutants were obtained through ultraviolet (UV) irradiation, culturing on zinc thiazole-amended nutrient agar (NA) plates, and culturing on zinc thiazole-treated rice plants. These zinc thiazole-resistant mutants had resistance factors (RF = EC_(50) value of a mutant / EC_(50) value of the wildtype parent of this mutant) of 12·4 to 1861 with a mean RF value of 441. Mutants obtained via UV irradiation, culturing on NA plates and culturing on rice plants had mean RF values of 51·8, 24·5 and 14·4, respectively. All mutants showed decreases in resistance to zinc thiazole after 20 successive transfers on bactericide-free media or 10 successive inoculation-reisolations on bactericide-free rice plants. No significant difference was found in bacterial growth and sensitivity to bismerthiazol between zinc thiazole-resistant mutants and their parents. However, a significant decrease was observed in the pathogenicity of zinc thiazole-resistant mutants compared with their parents, especially for mutants obtained via UV irradiation.
机译：在2009-2010年期间，共有323株米氏黄单胞菌（xanthomonas oryzae pv）分离株。稻米是从中国四个省（浙江，江苏，安徽和湖北）的具有细菌性叶枯病症状的水稻中获得的。测试了这些分离物对噻唑锌的基线敏感性，噻唑锌是一种对黄单胞菌具有强抗菌活性的新型杀菌剂。在整个两年的研究期内，所有四个地区的抽样病原菌种群对噻唑锌（OT-16-8 mg L〜（-1））的敏感性均相似。基线敏感性分布为单峰曲线，平均ECso值为6·79±1·61 mg L〜（-1）。水稻X. pv。中噻唑锌抗性突变的风险。稻米在体外和体内被进一步评估。通过紫外线（UV）辐照，在经噻唑锌修饰的营养琼脂（NA）平板上培养并在经噻唑锌处理的水稻植株上获得12个抗噻唑锌的突变体。这些抗噻唑锌的突变体的耐药因子（RF =突变体的EC_（50）值/该突变体的野生型亲本的EC_（50）值）为12·4至1861，平均RF值为441。通过紫外线照射，在NA平板上培养和在水稻植物上培养的平均RF值分别为51·8、24·5和14·4。在无细菌培养基上连续转移20次或在无细菌水稻植物上连续接种10次后，所有突变体均显示出对噻唑锌的抗性降低。耐噻唑锌的突变体与其父母之间在细菌生长和对比美噻唑的敏感性方面没有发现显着差异。然而，观察到耐锌噻唑的突变体与其亲本相比的致病性显着降低，特别是对于通过紫外线照射获得的突变体。

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