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首页> 外文期刊>Polish journal of veterinary sciences >Molecular analysis of the nucleoprotein gene of canine distemper virus isolated from clinical cases of the disease in foxes, minks and dogs.
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Molecular analysis of the nucleoprotein gene of canine distemper virus isolated from clinical cases of the disease in foxes, minks and dogs.

机译:从狐狸,水貂和狗的临床病例中分离出的犬瘟热病毒核蛋白基因的分子分析。

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摘要

In this study, we used RT-PCR to detect and characterize canine distemper virus isolated from 9 naturally infected foxes, 3 minks and 3 dogs in Poland by amplifying and sequencing a portion of the NP gene. A 293-bp fragment of the CDV NP gene was amplified by RT-PCR. Sequencing of the PCR products from the isolates led to the identification of 3 sequence variants. The mostly representative polymorphic variant No. 1 showed high homology with Chinese isolate of CDV with a accession number EF 375619. The sequences of all isolates from this polymorphic variants compared with the sequences of other polymorphic variants obtained in the study and with European and American isolates sequences from GenBank showed the conservative nucleotides changes in positions 57, 132, 143, 159 and 237. These mutations can indicate that in this part of Europe there are new variants of CDV.
机译:在这项研究中,我们通过扩增和测序NP基因的一部分,使用RT-PCR检测和鉴定了从波兰9只自然感染的狐狸,3只貂和3只狗中分离出的犬瘟热病毒。通过RT-PCR扩增了 CDV NP基因的293bp片段。从分离物中对PCR产物进行测序导致鉴定3个序列变体。最具代表性的1号多态性变体与中国CDV分离株(登录号EF 375619)具有高度同源性。与该研究中获得的其他多态性变体的序列以及欧洲和美国分离株的序列相比,该多态性变体的所有分离物的序列GenBank的序列显示第57、132、143、159和237位的保守核苷酸变化。这些突变表明在欧洲的这一地区有CDV的新变体。

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