A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines

Hu L.; Lin X. Y.; Yang Z. X.; Yao X. P.; Li G. L.; Peng S. Z.; Wang Y.

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A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines

机译：用于同时检测猪中经典猪瘟病毒，非洲猪瘟病毒，高致病性猪繁殖与呼吸综合征，猪繁殖与呼吸综合征和伪狂犬病的多重PCR

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In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09x10(4), 1.50x10(3), 2.10x10(3), 1.30x10(3) and 8.97x10(2) copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.

机译：在此分析中，我们开发并评估了多重PCR（mPCR）能够同时检测多种猪感染的能力。四对引物用于检测五种病毒。针对经典猪瘟病毒（CSFV），非洲猪瘟病毒（ASFV）和伪狂犬病（PRV）设计了特异性引物。为两种不同类型的猪生殖和呼吸综合症病毒（PRRSV），高致病性猪生殖和呼吸综合症病毒（HP-PRRSV）分别设计了一对引物。 mPCR的检出限为CSFV，ASFV，HP-PRRSV，PRRSV和CSFV，ASFV，HP-PRRSV和PRV，分别。通过mPCR检测了总共49个临床标本，结果表明，两种或三种病毒的共感染率为51％。总之，PCR不仅是猪单发感染而且是混合感染的临床诊断的有用工具。

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《Polish journal of veterinary sciences》 |2015年第4期|共9页
作者
Hu L.; Lin X. Y.; Yang Z. X.; Yao X. P.; Li G. L.; Peng S. Z.; Wang Y.;
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正文语种 eng
中图分类动物医学（兽医学）;
关键词
Multiplex PCR; ASFV; CSFV; PRV; PRRSV; HP-PRRSV;

机译：多重PCR;ASFV;CSFV;PRV;PRRSV;HP-PRRSV;

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1. A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines [J] . Hu L., Lin X. Y., Yang Z. X., Polish journal of veterinary sciences . 2015,第4期

机译：用于同时检测猪中经典猪瘟病毒，非洲猪瘟病毒，高致病性猪繁殖与呼吸综合征，猪繁殖与呼吸综合征和伪狂犬病的多重PCR
2. Development of a novel hot-start multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus [J] . Giammarioli M, Pellegrini C, Casciari C, Veterinary Research Communications . 2008,第3期

机译：用于同时检测古典猪瘟病毒，非洲猪瘟病毒，猪循环系统型2，猪生殖和呼吸综合征病毒和猪节目病毒的新型热启动多重PCR
3. Multiplex PCR for the simultaneous detection of porcine reproductive and respiratory syndrome virus, classical swine fever virus, and porcine circovirus inpigs [J] . LiuJ.-K., WeiC.-H., YangX.-Y., Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines . 2013,第3a4期

机译：多重PCR同时检测猪繁殖与呼吸综合征病毒，经典猪瘟病毒和猪圆环病毒猪
4. LUNG LESIONS IN PIGS VACCINATED WITH THE CLASSICAL SWINE FEVER VIRUS AND CHALLENGED WITH THE PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME [C] . Lozada A, Mendoza S, Correa P, Congress ofthe International Pig Veterinary Society . 2008

机译：猪的肺病灶与古典猪瘟病毒接种疫苗并挑战猪呼吸和生殖综合征
5. Investigation of the effects of porcine reproductive and respiratory syndrome virus (PRRSV) exposure dose on pregnant swine, the effect of PRRSV on the ovary, and methods for detection of PRRSV in the fetus. [D] . Benson, James Edwards. 2001

机译：猪繁殖与呼吸综合症病毒（PRRSV）暴露剂量对怀孕猪的影响，PRRSV对卵巢的影响以及胎儿PRRSV检测方法的调查。
6. Use of a Multiplex RT-PCR Assay for Simultaneous Detection of the North American Genotype Porcine Reproductive and Respiratory Syndrome Virus Swine Influenza Virus and Japanese Encephalitis Virus [O] . Hong-ying CHEN, Zhan-yong WEI, Hong-ying ZHANG, -1

机译：多重RT-PCR分析用于同时检测北美基因型猪繁殖与呼吸综合症病毒猪流感病毒和日本脑炎病毒的用途
7. Concurrent Porcine Reproductive and Respiratory Syndrome Virus,Porcine Circovirus Type 2 and Pseudorabies Virus InfectionsAssociatedWith ‘High Fever Syndrome’ in Swine of Northern China [O] . Xiaoling Wang, Feng Hu, Wencheng Lin, 2011

机译：并发猪生殖和呼吸综合征病毒，猪胃肠病毒2型和伪症病毒感染了北方猪的“高烧综合征”

A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines

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