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首页> 外文期刊>Polish journal of veterinary sciences >A comparison of the effectiveness of the microscopic method and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera) from winter hive debris
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A comparison of the effectiveness of the microscopic method and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera) from winter hive debris

机译：显微镜方法和多重PCR方法在鉴定和区分Nosema spp菌种中的有效性比较。冬季蜂巢碎片中的工蜂（Apis mellifera）中的孢子

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摘要

The objective of this study was to compare the effectiveness of the multiplex PCR method and traditional light microscopy in identifying and discriminating the species of Nosema spp. spores in worker bees from winter hive debris in the Province of Warmia and Mazury (NE Poland). A total of 1000 beesdead after from the bottom of the hive from bee colonies were analyzed. Spores were identified with the use of a light microscope (400-600x magnification). Spores were assigned to species by the multiplex PCR method. The microscopic evaluation revealed the presence of Nosema spp. spores in 803 samples (80.3%). Nosema ceranae spores were observed in 353 positive samples (43.96%), Nosema apis spores were found in 300 samples (37.35%), while 150 samples (19.67%) showed signs of a mixed infection. A multiplex PCR analysis revealed that 806 samples were infested with Nosema spp., of which 206 were affected only by Nosema ceranae, 600 showed signs of mixed invasion, while no samples were infected solely by Nosema apis parasites. In two cases, the presence of spores detected under a light microscope was not confirmed by the PCR analysis.The results of the study indicate that Nosema ceranae is the predominant parasitic species found in post-winter worker bees from the bottom of the hive in the region of Warmia and Mazury.
机译：这项研究的目的是比较多重PCR方法和传统的光学显微镜在识别和区分Nosema spp物种中的有效性。 Warmia and Mazury（东北波兰）的冬季蜂巢碎片中的工蜂孢子。从蜂巢的蜂巢底部总共分析了1000只蜜蜂。使用光学显微镜（400-600x放大倍率）鉴定孢子。通过多重PCR方法将孢子分配给物种。显微镜评估显示Nosema spp的存在。 803个样品中的孢子（80.3％）。在353份阳性样品（43.96％）中观察到了Nosema ceranae孢子，在300份样品（37.35％）中发现了Nosema apis孢子，而150份样品（19.67％）显示出混合感染的迹象。多重PCR分析显示，有806个样品被Nosema spp。侵染，其中206个仅受到了Nosema ceranae的影响，600个显示出混合入侵的迹象，而没有样品仅被Nosema apis寄生虫感染。在两种情况下，PCR分析未确认在光学显微镜下检测到的孢子的存在。研究结果表明，Nosema ceranae是冬季工蜂中从蜂巢底部发现的主要寄生物。 Warmia和Mazury地区。

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