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Comparative Quantitative Analysis of Artemisinin by Chromatography and qNMR

机译：青蒿素的色谱和qNMR比较定量分析

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摘要

Introduction - Since the discovery of artemisinin in the 1970s, many techniques based on diverse chromatography techniques have been developed to detect and quantify this important antiplasmodial compound. The accurate quantification of this compound in the Artemisia annua plant material is mainly needed for breeding purposes in order to cultivate higher yielding varieties. It is also important for the quality control of herbal preparations containing A. annua plant material.Objective - To evaluate the most common validated quantification techniques (LC-MS, HPLC-ELSD and TLC) and compare the results to quantitative nuclear magnetic resonance spectroscopy (qNMR) in eight different A. annua samples collected from around the world.Methodology - The leaf material were extracted according to standard procedures and analysed with the validated quantification techniques. For the qNMR analysis we did not employ a standard curve but instead used an internal standard (maleid acid) which is not chemically related to artemisinin.Results - We found a significant difference between the results in this study. Compared with the qNMR results the HPLC-ELSD corresponded closely, followed by LC-MS. Quantitation with TLC led to an estimation range of-0.5 to +3.2 mg artemisinin/g of A. annua.Conclusion - These results imply that qNMR, with the addition of an internal standard, can be used to quantify artemisinin in A. annua samples in a rapid and reproducible manner. Copyright
机译：简介-自1970年代发现青蒿素以来，已开发出许多基于多种色谱技术的技术来检测和定量这种重要的抗血浆化合物。为了培育更高产量的品种，主要需要在蒿属植物材料中对这种化合物进行准确定量，以用于育种目的。目的-对含有A.annua植物材料的草药制品的质量控制也很重要。目的-评价最常用的经过验证的定量技术（LC-MS，HPLC-ELSD和TLC），并将结果与​​定量核磁共振波谱法（方法）-根据标准程序提取叶片材料，并使用经过验证的定量技术进行分析。对于qNMR分析，我们没有使用标准曲线，而是使用了与青蒿素化学无关的内标（马来酸）。结果-我们发现这项研究的结果之间存在显着差异。与qNMR结果相比，HPLC-ELSD密切对应，随后是LC-MS。 TLC定量估计的青蒿素/ g值为-0.5至+3.2 mg青蒿素。结论-这些结果表明，qNMR和内标可用于定量青蒿素样品中的青蒿素。以快速且可复制的方式。版权

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