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首页> 外文期刊>Phytochemical Analysis >An Efficient Strategy based on MAE, HPLC-DAD-ESI-MS/MS and 2D-prep-HPLC-DAD for the Rapid Extraction, Separation, Identification and Purification of Five Active Coumarin Components from Radix Angelicae Dahuricae
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An Efficient Strategy based on MAE, HPLC-DAD-ESI-MS/MS and 2D-prep-HPLC-DAD for the Rapid Extraction, Separation, Identification and Purification of Five Active Coumarin Components from Radix Angelicae Dahuricae

机译：基于MAE，HPLC-DAD-ESI-MS / MS和2D-prep-HPLC-DAD的高效策略，用于从当归中快速提取，分离，鉴定和纯化5种活性香豆素成分

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Introduction - Further studies of active coumarin components in Radix Angelicae Dahuricae (AE) are absolutely essential to provide data on pharmacology, toxicology and quality for innovative drug candidates. Thus, the preparation of active component standards and the administration of coumarin monomers should be carried out. The isolation of the low-level active components from complex Traditional Chinese Medicine (TCM) samples necessitates the development of rapid, simple and economical modern extraction, separation, identification and purification methods.Objective - To develop an efficient strategy for the rapid extraction, separation, identification and purification of coumarins from AE.Methodology - First, active coumarins in AE were extracted with microwave-assisted extraction (MAE) after the extraction conditions were optimised. Second, gradient extraction methods with MAE were used to partially purify AE. Third, a high-performance liquid chromatography-diode array detection-electrospray ionisation tandem mass spectrometry (HPLC-DAD-ESI-MS/MS) method was applied for the preliminary on-line identification and screening of the main coumarins in AE extract. Finally, a two-dimensional preparative high-performance liquid chromatography-diode array detection (2D-prep-HPLC-DAD) system was developed for further preparative separation of those target components.Results - Altogether 10 coumarins have been identified and five of them including xanthotoxol, osthenol, oxypeucedanin hydrate, byakangelicin and imperatorin were deemed as target components for the preparative isolation. All of the five isolated coumarins were at high purities of over 99% and the production rate was much higher than the traditional methods.Conclusion - The present paper demonstrates that these consecutive approaches are very useful for to isolate chemical constituents from TCM. Copyright
机译：简介-当归中的活性香豆素成分的进一步研究对于为创新候选药物提供药理学，毒理学和质量数据至关重要。因此，应进行活性成分标准品的制备和香豆素单体的管理。从复杂的中药（TCM）样品中分离低含量的活性成分需要开发一种快速，简单且经济的现代提取，分离，鉴定和纯化方法。目的-为快速提取，分离制定有效的策略方法学-首先，在优化提取条件后，通过微波辅助提取（MAE）提取AE中的活性香豆素。其次，使用MAE进行梯度提取的方法可部分纯化AE。第三，采用高效液相色谱-二极管阵列检测-电喷雾电离串联质谱（HPLC-DAD-ESI-MS / MS）方法对AE提取物中的主要香豆素进行初步的在线鉴定和筛选。最后，开发了一种二维制备型高效液相色谱-二极管阵列检测（2D-prep-HPLC-DAD）系统，用于进一步分离这些目标成分。结果-共鉴定出10种香豆素，其中包括5种香豆素。氧杂草醇，邻苯二酚，水合氧化皮斯丹宁，拜金奇霉素和欧前胡素被认为是制备性分离的目标组分。五种分离出的香豆素均具有超过99％的高纯度，产率远高于传统方法。结论-本文证明了这些连续的方法对于从中药中分离化学成分非常有用。版权

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