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首页> 外文期刊>Phytochemical Analysis >Effects of Auxins on the Production of Steroidal Alkaloids in Rapidly Proliferating Tissue and Cell Cultures of Solanum lyratum
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Effects of Auxins on the Production of Steroidal Alkaloids in Rapidly Proliferating Tissue and Cell Cultures of Solanum lyratum

机译：生长素对快速增生白果茄组织和细胞培养中甾体生物碱产生的影响

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Introduction Solanum lyratum, a rare species, is used to treat cancer, tumours and warts. Plant cell and tissue culture of S. lyratum, producing steroidal alkaloids, could be useful supplements to natural sources. Objective To study the production of solanine, solanidine and solasodine by adding auxin-type phytohormones including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) to cell and callus cultures of S. lyratum. Methodology Methanolic extracts were made from callus and cell cultures of S. lyratumand and analysed using RP C18 HPLC with UV detection. Results 2,4-D-induced calli from roots led to a significant enhancement in solanine production with a value of 4.13?mg/g dry weight (DW). The maximal solanidine and solasodine levels of 6.26 and 7.69?mg/g DW were respectively obtained with IBA- and IAA-treated S. lyratum cells at concentrations of 1 and 5?mg/L. Conclusion Auxins were found to be useful phytohormones for the production of steroidal alkaloids. The callus and cell culture system developed is simple and can hence be a method of production of steroidal alkaloids in S. lyratum and other Solanaceae species. Copyright (C) 2011 John Wiley & Sons, Ltd.
机译：引言茄属稀有种被用于治疗癌症，肿瘤和疣。拟南芥的植物细胞和组织培养产生甾体生物碱，可能是天然来源的有益补充。目的研究添加吲哚-3-乙酸（IAA），吲哚-3-丁酸（IBA），萘乙酸（NAA）和2,4-二氯苯氧基乙酸等生长素型植物激素来生产茄碱，茄碱和茄索定（2,4-D）到S. lyratum的细胞和愈伤组织培养物中。方法学从山茱。的愈伤组织和细胞培养物中提取甲醇，并使用RP C18 HPLC和UV检测进行分析。结果2，4-D诱导的根部愈伤组织导致茄碱产量显着提高，其值为4.13？mg / g干重（DW）。用IBA和IAA处理过的S. lyratum细胞分别以1？5？mg / L的浓度分别获得6.26和7.69？mg / g DW的最大茄碱和solaso​​dine水平。结论发现生长素是类固醇生物碱的有用植物激素。开发的愈伤组织和细胞培养系统很简单，因此可以成为在美白葡萄球菌和其他茄科植物中生产甾体生物碱的一种方法。版权所有（C）2011 John Wiley＆Sons，Ltd.

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